摘要
研究目的:通过分析miRNA的核心启动子和顺式作用元件为进一步解析大豆(Glycine max)miRNAs表达调控及其功能研究提供重要信息。创新要点:利用生物信息学方法全面解析了大豆降解组文库miRNA的启动子特征,并依据顺式作用元件及靶基因构建了miRNA的表达与生长素响应因子、赤霉素响应因子之间存在潜在的负反馈调控网络。研究方法:本研究利用TSSP程序和PlantCARE数据库预测了来自大豆降解组文库的440个miRNA的核心启动子以及369个miRNAs的顺式作用元件,并依据顺式作用元件及靶基因构建miRNA调控网络。重要结论:83.86%的miRNA在其上游序列中含有启动子,8.64%的miRNA在其下游序列中含有启动子,21.59%的miRNA包含增强子。核心启动子的TATA盒与转录起始位点(TSSs)的分布相似。此外,对转录起始位点5'端的顺式作用元件预测为miRNAs的可能功能和表达的时空性提供了线索。miRNAs的顺式作用元件和靶基因的分析显示,部分miRNA的表达与生长素响应因子、赤霉素响应因子之间存在潜在的负反馈调控。
Objective： MicroRNAs （miRNAs） are genome-encoded, small non-coding RNAs that play important functions in development, biotic and abiotic stress responses, and other processes. Our aim was to explore the regu- lation of miRNA expression. Methods： We used bioinformatics methods to predict the core promoters of 440 miRNAs identified from a soybean （Glycine max） degradome library and to analyze c/s-acting elements for 369 miRNAs. Results： The prediction results showed that 83.86% of the 440 miRNAs contained promoters in their upstream se- quences, and 8.64% （38 loci） in their downstream sequences. The distributions of two core promoter elements, TATA-boxes and transcription start sites （TSSs）, were similar. The cis-acUng elements were examined to provide clues to the function and regulation of spatiotemporal expression of the miRNAs. Analyses of miRNA cis-elements and targets indicated a potential auxin response factor （ARF）- and gibberellin response factor （GARF）-mediated negative feedback loop for miRNA expression. Conclusions： The features of miRNAs from a Glycine max degradome library obtained here provide insights into the transcription regulation and functions of miRNAs in soybean.
基金
supported by the National Natural Science Foundation of China(No.31271652)
the National Science Foundation for Young Scientists of China(No.31301708)
