黄芪注射液对大鼠脑缺血再灌注损伤的保护作用研究

The protective effect of astragalus injection in cerebral ischemia reperfusion injury in rats

目的探讨黄芪注射液对大鼠脑缺血/再灌注损伤的保护作用和机制。方法应用线栓法经左侧颈外-颈内动脉插线建立大鼠大脑中动脉阻塞再灌注(MCAO/R)模型,经腹腔注射黄芪注射液(3 ml/kg)干预治疗。Longa法评价大鼠神经行为功能,氯化三苯基四氮唑(TTC)染色观察脑梗死体积,苏木精-伊红(HE)染色观察海马神经元形态结构,TUNEL法检测细胞凋亡,免疫组化检测c-jun氨基末端激酶(JNK3)蛋白表达水平。结果经黄芪注射液治疗后大鼠海马神经元JNK3蛋白表达水平显著降低,凋亡细胞数量显著减少,神经元形态恢复,脑梗死体积缩小,大鼠神经行为功能显著改善。结论黄芪注射液可通过下调JNK3表达水平而抑制细胞凋亡,缩小脑梗死体积,改善大鼠神经行为功能。

Objective To study the protective effect of astragalus injection in cerebral isehemia reperfu sion injury in rats. Methods The middle cerebral artery occlusion reperfusion （MCAO/R） rat models were es tablished by inserting a filament suture from left externalinternal carotid artery, and treated by injecting intra peritoneally astragalus injection （3 ml/kg）. The neurobehavioral function was evaluated by Longa＇s test, the cerebral infarct volume stained By tetrazolium chloride （TTC） assay, and the structure of cells observed with hematoxylineosin staining. The neuronal apoptosis and the expressions of cJun Nterminal kinase （JNK） were respectively detected by terminal deoxynucleotidyl transference mediated biotinylated deoxyuridine triphosphate nick end labeling （TUNEL） and immunohistochemieal assay. Results After treated by astragatus injection, the expression of JNK3 in hippocampus, the number of apoptotic neurons and the cerebral infarction volume reduced significantly than those in model group. The shapestructure of neurons and neurobehavioral function of rats improved than those in model group. Concliom Astragalus injection might improve the neu robehavioral function of rats by downregulating the expression of JNK3 and inhibiting neuronal apoptosis in hippocampus.