Na_2CO_3胁迫对番茄叶片的渗透调节效应的研究

Influence of Na_2CO_3 Stress on Osmotic Adjustment of Tomato Leaves

[目的]研究盐胁迫下番茄生长发育规律和耐盐机理,为选育耐盐番茄品种提供理论依据。[方法]利用0、10、20、40、80 mmol/L5个浓度Na2CO3胁迫佳粉17、月光、L-402 3种番茄,以0 mmol/L为对照。采用随机区组设计,设15个小区,每个小区5盆,重复3次。[结果]随着盐处理浓度的增加,样品番茄叶片内脯氨酸、可溶性糖、电导率(EC)等生理生化指标均呈现显著增加的趋势。在同一胁迫条件下,脯氨酸、可溶性糖和电导率增加量的顺序为L-402>佳粉17>月光。[结论]盐胁迫下番茄叶片体内脯氨酸和可溶性糖小分子渗透调节物质增多,番茄体内膜透性增加,选择性降低,质膜受伤害的程度越大,植物抗逆性越小。番茄适合盐碱地种植的顺序为月光、佳粉17、L-402。

[ Objective ] The rule of growth and development of tomato under salt stress and salt tolerance mechanism were studied to provide theoretical basis for breeding salt tolerant tomato varieties. [ Method] By 0, 10, 20, 40, 80 mmol/L Na2CO3 stress, good powder 17, moon-light and L-402 were taken as materials, 0 mmol/L as control for each variety. Randomized block design, with 15 districts, each district 5 pots, repeat 3 times were conducted. [ Result] With the increase of salt concentration, the proline, soluble sugars and conductivity in tomato leaf samples showed a significant increasing trend. Under the same stress, the increase of proline, soluble sugars and conductivity was in the order of L-402, Jiafen 17 and moonlight. [ Conclusion] Under the salinity stress, the osmotic adjustment materials of small molecule of both proline and soluble sugars in tomato leaves increased, the membrane permeability increased, while the selectivity decreased. The more the plasmalemma being damaged, the less stress resistance the plant got. The tomato sample suitable for planting in saline-alkali soil was in the or-der of moonlight, Jiafcn 17 and L-402.