VEGF在伴鼻息肉的慢性鼻-鼻窦炎中的表达及克拉霉素对其的调节作用

The expression of VEGF and the regulation of clarithromycin on it in chronic rhinosinusitis with nasal polys

目的:探讨血管内皮生长因子(VEGF)在伴鼻息肉的慢性鼻-鼻窦炎(CRSwNP)患者鼻黏膜中的表达及克拉霉素对其的调控作用。方法:收集16例CRSwNP组织及21例单纯鼻中隔偏曲患者下鼻甲黏膜,采用实时定量RT-PCR方法检测样本中VEGF的表达。另外收集10例伴鼻息肉的慢性鼻窦炎鼻息肉组织及5例单纯鼻中隔偏曲患者下鼻甲黏膜,采用体外组织块培养及ELISA方法,观察克拉霉素对VEGF表达的调节作用。结果:①VEGF mRNA在CRSwNP组的表达较对照组增高,差异有统计学意义(P<0.01);②经克拉霉素刺激前后比较,CRSwNP组织中VEGF蛋白的表达量下降,差异有统计学意义(P<0.05)。结论:CRSwNP组织中VEGF的表达显著升高,推测VEGF在鼻息肉的发病机制中具有极其重要的作用。克拉霉素可能通过抑制VEGF的表达而达到治疗慢性鼻-鼻窦炎的作用。

Objective：To investigate the expression of vascular endothelial growth factor in the nasal mucosa of chronic rhinosinusitis with nasal polys patients, and explored the regulation of clarithromycin on VEGF. Meth- od..RT-PCR was used to detect the expression of VEGF in nasal mucosa from healthy control and CRSwNP. Nasal mucosal tissue explant culture measure and ELISA were used to explore the effect of clarithromyein on VEGF ex- pression. Result：OVEGF mRNA expression level was significantly increased in CRSwNP compared with control and showed a statistic difference（P〈0.01）. QThere was a significant decrease in CRSwNP group undergo clar- ithromycin treatment on protein expression level of VEGF and showed a statistic difference（P〈0.05）. Conclusion： VEGF were overexpressed in CRSwNP group, which presume that play an important role in the pathogenesis of nasal polyps. Clarithromycin may play a therapeutical role on chronic rhinosinusitis through down-regulated the ex- pression of VEGF.