变应性鼻炎患者外周血中神经营养因子mRNA的表达

Increase expression of neurotrophins mRNA in peripheral blood of patients with allergic rhinitis

目的:检测变应性鼻炎(AR)患者外周血中神经生长因子(NGF)、脑源性神经营养因子(BDNF)、神经营养蛋白-3(NT-3)mRNA的表达水平,分析基因表达与AR病情严重程度的关系。方法:采用成组对照试验,抽提AR患者外周血总RNA,以正常成年人为对照。使用实时定量RT-PCR检测NGF、BDNF、NT-3mRNA的表达水平,2-△△Ct法计算AR患者基因表达的变化倍数。结果:相较于正常成年人,AR患者外周血中NGF、BDNF、NT-3mRNA的2-△△Ct值依次为2.436 8、4.458 8、1.781 8;NT-3mRNA表达的2-△△Ct值随着AR严重程度加重呈递增趋势。结论:AR患者外周血中NGF、BDNF、NT-3mRNA的表达量上调,可能与AR的发病有关;NT-3可作为评价AR严重程度的分子生物学指标。

Objective： To assess the expression of NGF, BDNF, NT-3 mRNA in the peripheral blood of pa- tients with allergic rhinitis（AR）. Meanwhile, to analysis whether the expression of NGF, BDNF,NT-3 mRNA correlate with the severity of rhinitis. Method：This study is a group controlled trial, which takes the healthy adults as control group. The total RNA have been extracted from the peripheral blood of AR patients. The expression of NGF, BDNF and NT-3 mRNA have been tested by real-time quantitative RT-PCR. Result： Comparing with the healthy adults, the expression of NGF, BDNF and NT-3 mRNA as 2-/△△Ct are 2.436 8, 4. 458 8 and 1. 781 8 re- spectively. The increasing expression of NT-3 correlated positively with the scores of visual analog scale. Conclu- sion..The expression of NGF, BDNF and NT-3 mRNA are as high as 2. 4368, 4. 4588 and 1. 7818 times to healthy adults. We propose NGF, BDNF and NT-3 may contribute to the pathogenesis of AR. NT-3 could reflect the se- verity of rhinitis as a molecular biological index.