茶树多态性SSR分子标记引物筛选

Screening of Tea Polymorphic Primers of SSR Molecular Markers

本实验采用国家鉴定茶树品种丹桂自然杂交F1代12个新品(株)系DNA为模板,对60对茶树SSR分子标记引物进行筛选,其中12对引物PCR产物电泳结果较为理想,条带清晰、多态性丰富。其他48对引物没有多态性,或者条带模糊无法统计,或者扩增不出任何条带。从已筛选出的多态性引物中随机挑选2对引物D02、D08,对丹桂自然杂交FI代59个新品(株)系做PCR扩增,电泳检测能获得条带清晰、多态性丰富的胶图,说明筛选获得的12对多态性SSR引物可用于遗传多样性或亲缘关系等分子生物学实验。

In this study, 60 pairs of primers of tea SSR molecular markers were screened with the DNA templates of 12 new lines from FI natural hybrids of national tea cultivar Dangui. Electrophoresis of PCR products were found that about 12 pairs of SSR primers had ideal results with clear bands and rich polymorphism. The remaining 48 pairs of SSR primers were without polymorphic bands or with the vague bands can not be statistics, or the absence of any amplified bands. 59 new lines from FI natural hybrids of national tea cultivar Dangui were amplified with two pairs of primers of D02 and D08 randomly selected from the 12 pairs of polymorphic primers. Electrophoresis of PCR products can get clear bands and rich polymorphism gel figure, which illustrating 12 pairs of polymorphic SSR markers can be used for genetic diversity or phylogenetic relationships of molecular biology experiments and so on.