锯缘青蟹细菌性传染病的病原菌研究

Studies on the Pathogen of the Bacteria Epidemic against Mud Crab Scylla serrata

从宁波市锯缘青蟹 (Scyllaserrata)主要养殖海区 5～ 9月间不同症状的病蟹体内分离到 6株细菌 ,S990 1、S990 2、S990 3、S990 4、S990 5和S990 6 ,经感染试验确定S990 2、S990 3、S990 5和 990 6均为病原菌 ;此 4株菌革兰氏染色阴性 ,具 1根极生鞭毛 ,氧化酶反应阳性 ,发酵葡萄糖 ,不产气 ,不发酵肌醇 ,对 0 / 12 9试剂敏感 ,为弧菌属种类 ;经赖氨酸、精氨酸、鸟氨酸脱羧酶、蔗糖、甘露醇发酵、 6 %氯化钠胨水生长、水杨素、枸橼酸盐利用等试验 ,根据第二代GYZ— 9V系统 ,分别将其归为辛辛那提弧菌、溶藻弧菌和副溶血弧菌。 4株菌对氯霉素、环丙沙星和硫酸庆大霉素等抗菌素表现出强敏感性 ,诺氟沙星、磺胺甲异恶唑等中度敏感 ,而对头咆唑啉钠、呋喃唑酮不敏感 ;中草药五倍子和五味子煎出液对 4株菌均表现出较强的抑菌性能。

During May to September in 1999,six strains were isolated from diseased mud crabs with different symptom,which were cultured in marine ponds of various district in Ningbo.The strains were named as S9901、S9902、S9903、S9904、S9905 and S9906 S9902、S9903、S9905 and S9906 were proved to be pathogenic through artificial intramuscularly injection And the morphologic and biochemical tests showed that those strains were all Gram negative,with one flagella,oxidase positive,glucose fermentation positive,inositol negative,sensitive to 0/129 reagent(150μg) Based on these properties,they were assigned to Vibrio genus.Other nine biochemical tests were carried out,and based on the results,the four strains were identified to Vibrio cincinnatiensis,V.alginolyticus,V.parahemolyticus and V.parahemolyticus. The four strains are sensitive to Chloramphenicol,Ciprofloxacin,and Gentamycin.Chinese herb medicine, Fructus schizandrae and Galla chinesis also showed good antibiotic performance against the strains.