摘要
目的 :观察滋肾活血解毒方对慢性肾衰竭 (CRF)大鼠残肾组织中ET 1mRNA的影响。方法 :采用 5/ 6肾切除法复制大鼠CRF模型 ,将 50只Wistar大鼠随机分成对照组、保肾康组、洛汀新组、滋肾活血解毒方组 (观察组 )、正常组 ,用RT PCR技术检测各组大鼠残肾组织中ET 1mRNA表达水平。结果 :正常组肾组织中ET 1mRNA表达最弱 (P <0 .0 1) ,对照组肾组织中ET 1mRNA表达最明显 (P <0 .0 1) ,观察组肾组织中ET 1mRNA表达较保肾康组及洛汀新组有明显下降 (P <0 .0 1)。结论 :滋肾活血解毒方可能是通过下调肾脏组织中ET 1mRNA表达水平而改善CRF大鼠肾功能。
Objective:To explore effects of Zishen Huoxue Jiedu recipe(ZSHXJDR) on the mRNA expression of endothelin-1(ET-1)in remnant kidney of rats with chronic renal failure(CRF).Method:Rats were divided into five groups:ZSHXJDR group,lotensin group,Baoshenkang group(BSK),control group,normal control group.CRF animal model of rats were established after 5/6 of kidney mass were removed.The mRNA expression levels of ET-1 in remnant kidney were measured by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR)。Result:The ET-1 mRNA expression levels in remnant kidney of control group was significantly higher than that of normal control group,ZSHXJDR group,lotensin group,and BSK group.The ET-1 mRNA expression levels in remnant kidney of ZSHXJDR was significantly lower than that of lotensin group,BSK group.Conclusion:ZSHXJDR can markedly improved renal function by down-regulating ET-1mRNA expression levels,reducing glomerular sclerosis.
出处
《安徽中医学院学报》
CAS
2001年第1期40-42,共3页
Journal of Anhui Traditional Chinese Medical College
