IL-6在U937细胞中特异、持续激活STAT3

IL-6 selectively activates STAT3 in U937 cells

目的探讨ＩＬ－６在Ｕ９３７细胞中产生生物学效应的信号转导基础。方法检测ＩＬ－６对Ｕ９３７细胞生长与分化的影响，并采用凝胶阻滞电泳法，检测ＩＬ－６在Ｕ９３７细胞中激活核因子的情况。结果ＩＬ－６可诱导Ｕ９３７细胞向巨噬细胞方向分化，分化的细胞酸性醋酸酯酶（ＡＮＡＥ）活性、硝基蓝四唑（ＮＢＴ）还原能力及ＣＤ５４表达增强。ＳＴＡＴ３可被ＩＬ－６显著激活，其活性呈一定的剂量与时间依赖性；而ＮＦ－ＩＬ６、ＮＦ－ｋＢ、ＡＰ－１及其它ＳＴＡＴ家族成员则无明显激活。结论Ｕ９３７细胞的终末分化可能是ＪＡＫ－ＳＴＡＴ３通路介导的。

Aim To investigate the signaling basis of the biological effects of IL-6 in U937 cells. Methods We determined the effect of IL-6 on the growth and differentiation of U937 cells and the effect of IL-6 on the activation of nuclear factors in U937 cells. Results IL-6 induced macrophage differentiation of U937 cells. Differentiated cells had strengthened ANAE (acid naphthyl acetate esterase) and NBT(nitroblue tetrazolium) -reducing activities and CD54 expression was upregulated. STAT3 could be remarkably activated by IL-6 in U937 cells and STAT3 activity was dependent on dose and time course of IL-6 treatment. However, NF-IL6, NF-kB, AP-1 and other STAT members were not activated by IL-6. Conclusion IL-6 might induce terminal differentiation of U937 cells via JAK-STAT3 pathway.