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Cdk5在肾小球足细胞中的表达及作用 被引量：3

Expression and function of Cdk5 in glomerular podocyte
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摘要 目的研究周期素依赖性蛋白激酶-5(Cdk5)及其激活剂p35在肾小球足细胞中的表达及作用。方法分别培养原代小鼠神经细胞、原代小鼠分化成熟的离体足细胞(简称足细胞)以及人胚肾细胞(HEK293细胞),应用免疫印迹、免疫沉淀及同位素标记等方法检测Cdk5、p35的表达及Cdk5的活性;以原代小鼠分化成熟的离体足细胞为研究对象,分为正常组、ROS组(Cdk5活性抑制组)、Cdk5 siRNA组(Cdk5表达沉默组),观察Cdk5的表达及活性,并应用Cdk5 siRNA、Cdk5化学抑制剂-Roscovitine进行抑制实验。结果离体培养原代小鼠分化成熟的足细胞中均有Cdk5及p35蛋白的表达;Cdk5在足细胞中具有活性;沉默Cdk5表达、应用Cdk5活性抑制剂Roscovitine抑制Cdk5活性后,可引起足细胞特异性标志物WT1的表达减少,说明足细胞受到损伤,数量减少。结论 Cdk5的表达及活性在维持正常足细胞功能中起重要作用。 Objective To study the expression and function of Cdk5 and its activator p35 in glomerular podocyte. Methods Primary culture of cortical neurons,podocyte,and HEK293 cells of mouse in in vitro,and the expression of Cdk5 were detected,p35 was detected by u- sing western blot and immunofluorescence staining,and the activity of Cdk5 using immune precipitation and isotope labeling. Results There were proteins p35 and Cdk5 expressed in the podocyte of mouse primarily cultured in vivo; p35 could active Cdk5 ; silencing the expression of Cdk5 or inhibition of Cdk5 activity using roscovitine （a Cdk5 inhibitor） could reduce the expression of WT1 ,a specific marker in podocyte, which indicates the podocyte damage and cell decreases. Conclusion The expression and activity of Cdk5 play an important role in maintai- ning normal functions in podosyte.
作者 毕逢辰 张彬 郑亚莉
机构地区 宁夏医科大学研究生院 宁夏人民医院肾脏内科
出处 《宁夏医学杂志》 CAS 2014年第3期193-195,I0001,共4页 Ningxia Medical Journal
基金 国家自然科学基金资助项目(NSFC81060066) 宁夏自然科学基金资助项目(NZ10162) 宁夏科技攻关资助项目(KGX1910-16) 宁夏科技攻关国际合作项目(2011ZYH169)
关键词 周期素依赖性蛋白激酶-5 P35 足细胞 SIRNA Cdk5活性 Cdk5 p35 Sertoli cell Sirna Cdk5 activity
分类号 R692 [医药卫生—泌尿科学]
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参考文献9

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二级参考文献34

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共引文献3

同被引文献17

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宁夏医学杂志
2014年 第3期

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