Fas与体外胸腺细胞自发凋亡的关系

The relationship between Fas expression and thymocytes spontaneous apoptosis in vitro

目的探讨体外培养的胸腺细胞表达Ｆａｓ、Ｆａｓ－Ｌ与自发凋亡的关系。方法应用流式细胞术，检测体外培养不同时间的胸腺细胞的凋亡率及Ｆａｓ、Ｆａｓ－Ｌ表达的阳性率。结果体外培养的胸腺细胞自发凋亡率和Ｆａｓ表达阳性率，均呈时间依赖性增加， Ｆａｓ－Ｌ在体外培养２４ ｈ时明显增加。体外培养的胸腺细胞Ｆａｓ的表达与自发凋亡明显相关。结论Ｆａｓ是介导体外培养的胸腺细胞自发凋亡的重要分子。

Aim To study the relationship between the expressions of Fas, Fas-L and the spontaneous apoptosis of thymocytes in vitro. Methods The expressions of Fas, Fas-L and the apoptotic rate of thymocytes were assayed by now cytometry. Results The apoptosis and the Fas expression of thymocytes of fifferent culture times in vitro were increased time-dependently in vitro. The Fas-L expression of thymocytes cultured for 24 h was also significant increase. There was significant corelation between the increase of Fas expression and the increase of apoptosis of thymocytes,in vitro. Conclusion Fas is an important molecule which mediates spontaneous apoptosis of thymocytes cultured in vitro.