非何杰金恶性淋巴瘤病人血清及瘤组织中β-葡萄糖醛酸酶同步测定及意义

The Synchronous Detection and Significance of β- Glucuronidase Both in Serum and Tumorous Tissue in Non Hodgkin Malignant Lymphoma Patient

目的 :探索非何杰金恶性淋巴瘤病人体内 β-葡萄糖醛酸酶 (β- glucuronidase,β- G)的变化规律。方法 :采用酶联免疫吸附测定 (enzym e- linked imm unsorbent assay,EL ISA)方法 ,免疫组织化学方法对 13例非何杰金恶性淋巴瘤患者血清及瘤组织中 β- G进行同步测定 ,并对其中 3例进行了免疫电镜研究。结果 :β- G在非何杰金恶性淋巴瘤患者血清及瘤组织中均呈明显表达 ,与对照组有显著性差异 (P <0 .0 1)。结论 :对 β- G进行机能学与形态学的联合检测 。

Objective: Our purpose was to explore the change regularity of β glucuronidase (β G) in body of patients with Non Hodgkin malignant lymphoma. Methods: β G was examined synchronously both in the serum and in the tumor tissue of 13 cases patient with Non Hodgkin malignant lymphoma by using the method of enzymlinked immunsorbent assay (ELISA) and immunohistochemistry separately. Among them, 3 cases were studied by using the immuno electron microscopic technique. Results: β G was highly expressed both in the serum and tumorous tissue in patients with non Hodgkin malignant lymphoma and there was obviously difference as compared with the control group (P<0.01). Conclusion: The combined detection with functional and morphological methods to β G, it may be assistant target to early discovery and early diagnosis of Non Hodskin malignant lymphoma.