胰腺癌MTS1/p16基因纯合性缺失和突变的研究

Deletion and Mutation of MTS1/p16 Gene in Human Pancreatic Carcinoma

目的 :研究人胰腺癌组织及正常胰腺组织中 p16基因第 1外显子和第 2外显子的纯合性缺失和突变情况 ,探讨 p16基因异常与胰腺癌的关系。方法 :应用聚合酶链式反应 -单链构象多态性分析 (PCR- SSCP)技术检测 5 1例胰腺癌组织中 p16基因第 1外显子和第 2外显子的纯合性缺失和突变频率 ,以正常胰腺组织 15例为对照。结果 :全部被检胰腺癌组织中有 2 3例发生 p16基因第 2外显子缺失 ,缺失率 45 % ,有 1例发生第 1外显子的突变 ,未发现第 1外显子缺失和第 2外显子突变。正常胰腺组织中均未发生第 1外显子和第 2外显子的缺失及突变。结论 :p16基因缺失与胰腺癌的发生关系密切 。

Objective: Our aim was to investigate the alteration of p16 gene in human pancreatic carcinoma. Methods: A total of 66 human pancreatic tissue specimens, comprising 51 with pancreatic carcinomas and 15 normal pancreatic tissue specimens, were examined for homozygous deletion and mutation of p16 gene by using PCR SSCP method. Results: No mutation and deletion was detected in 15 normal pancreatic tissue samples. Of 51 pancreatic carcinoma specimens, only one was found mutation for p16 gene in PCR SSCP assay, and the deletion of the p16 gene in 23 samples were confirmed by using PCR, with a 45% p16 gene deletion rate. Conclusion: These data suggest that p16 gene alterations may play a role in the progression of human pancreatic carcinoma.