非小细胞肺癌组织中上皮型钙粘蛋白的定量检测及临床意义

Quantitative Analysis of E-Cadherin Expression and Clinicopathologic Evaluation in Lung Cancer

目的 :探讨钙依赖性上皮细胞粘附分子即上皮型钙粘蛋白 (E- cadherin,EC)的表达和肺癌生物学行为的关系及临床意义。方法 :应用流式细胞术和细胞免疫荧光技术定量检测 39例手术切除非小细胞肺癌标本中 EC的表达和 DNA倍体分析 ,结合肺癌病理类型 ,探讨其与肺癌分化程度 ,TNM分期之间的关系。结果 :非小细胞肺癌EC表达量与正常肺组织相比差异显著 (P <0 .0 1)。在肺鳞癌中 ,低分化 ,纵隔淋巴结有转移者 EC表达量降低明显 (P <0 .0 1)。异倍体肺鳞癌 EC表达量较二倍体肺鳞癌低 (P <0 .0 5 )。 期的肺鳞癌 EC表达量较 , 期者降低 (P <0 .0 5 )。结论 :肺鳞癌中 EC的表达量下调分化程度较低 ,有淋巴结转移的趋势。肺腺癌中 EC表达下调与其分化程度 ,TNM分期 ,淋巴结转移和

Objective:Our aim was to evaluate the relationship of E cadherin(EC) expression with the biological behavior of lung cancer.Methods:The EC expression and the cellular DNA ploidy were measured using flow cytometry and immunofluorescence methods in 39 cases of primary non small cell lung cancer. Systemic pathological examination were performed for all cases.Results:The EC expression significantly reduced in all cases of lung cancer.Fluorescene index(FI) of EC expression was (0.90±0.10) in normal lung tissue, (0.49±0.09) in squamous cell lung cancer,(0.63±0.08) in adenocarcinoma.The expression of EC in squamous lung cancer greatly decreased in poor differentiation and with lymph node metastasis (P<0.01).In squamous cell lung cancer,the expression of EC was lower in uneuploid cancer than that in diploid cancer (P<0.05).The expression of E Cadherin in squamous cell lung cancer reduced more in TNM Ⅲ than in TNM Ⅰ,Ⅱ.Conclusion:The expression of EC decreased in poor differentiation squamous cell lung cancer with lymph node metastasis. In lung adenoma, the decrease in the expression of EC has no relatoin with the TNM stage, lymph node metastasis, and DNA ploidy.