柚皮苷对四氯化碳致亚急性肝纤维化的保护作用

Protective effects of Naringin on hepatic fibrosis in mice

目的探讨柚皮苷对四氯化碳(CCL4)致小鼠亚急性肝纤维化损伤的保护作用。方法小鼠经皮下多次注射CCL4进行亚急性肝纤维化模型的造模,首次注射CCL4的次日进行分组给药:柚皮苷高剂量(30mg/kg)和低剂量(15mg/kg)组,以醋酸地塞米松(2mg/kg)做阳性对照药。给药4周后断颈处死小鼠,分离肝脏、脾脏、胸腺并称重,计算小鼠肝脏、脾脏、胸腺的脏器系数。分离血清检测丙氨酸氨基转移酶(ALT)/谷丙转氨酶(GPT)、天门冬氨酸氨基转移酶(AST)/谷草转氨酶(GOT)、碱性磷酸酶(AKP)及肝组织中的羟辅氨酸(Hyp)含量。结果多次皮下注射CCL4可明显增加肝脏系数,血清AST/GOT、ALT/GPT、AKP含量以及肝脏羟脯氨酸的含量也增加,显示肝脏有纤维化损伤。柚皮苷高、低剂量均可明显降低四氯化碳导致肝脏系数的升高,同时降低血清AST/GOT、ALT/GPT、AKP含量以及肝脏羟脯氨酸的含量。结论柚皮苷对CCL4所致的小鼠亚急性肝纤维化有一定的保护作用。

Objective To investigate the protective effect of Naringin against carbon tetrachloride （CCL4）-induced hepatic fibrosis in mice. Methods The experimental model of hepatic fibrosis was induced by CCL4 in mice. KM mice were randomly divided into five groups： the normal group, the model group, high dose of Naringin treatment group （30mg/kg） ,low dose of Naringin treatment group（15 mg/kg） and dexamethasone treatment group（2mg/kg）. After 4 weeks, the serum AST/GOT, ALT/GPT, AKP, hepatic Hydroxyproline（Hyp） and liver, spleen and thymus index were determined. Results Liver index was increased by CCL4, and serum ALT/GPT, AST/GOT, AKP, Hyp were al- so increased in model group. The increased serum ALT/GPT, AST/GOT, AKP, Hyp and liver index were reduced by Naringin significantly. Conclusion Naringin has a protective effect on hepatic fibrosis induced by CCL4 in mice.