醒脑静对急性脑梗塞患者细胞粘附分子及T细胞亚群的影响

Effects of Xingnaojing Injection on Cell Adhesion Molecules and T Cell Subset in Patients with Acute Cerebral Infarction

观察醒脑静注射液治疗急性脑梗塞患者细胞粘附分子CD11b、CD54及T细胞亚群的变化。治疗前后取 2mL抗凝血 ,用荧光活化的细胞分析 (RACS)测定人血中细胞粘附分子CD11b、CD54及T细胞亚群CD4、CD8及其比值的变化。结果显示在急性脑梗塞中治疗前CD11b、CD54表达明显增多 ,而CD4、CD8的表达减少 ,CD4 /CD8比值降低。经醒脑静治疗 2周后 ,CD11b、CD54表达明显减低 ,而CD4、CD8的表达有所恢复 ,CD4 /CD8比值亦相对增高。说明醒脑静注射液治疗急性脑梗塞 ,减少脑损伤可能是与减少粘附因子CD11b、CD54的表达有关 ,同时可促使CD4、CD8比率增加 ,恢复免疫功能。

The changes of CD11b, CD54 and T cell subset were observed in the patients with acute cerebral infarction treated with Xingnaojing Injection (XI). 2 Milliliters of blood drawn from the patients and treated with anticoagulant before and after the XI treatment, was used to determine the changes of cell adhesion molecules CD11b and CD54, and CD4 and CD8 in the T cell subset by RACS method. The results showed that before the XI treatment, the expressions of CD11b and CD54 were obviously increased, those of CD4 and CD8 were decreased, and the ratio of CD4 +/CD8 + was lowered; after the XI treatment for 2 weeks, the expressions of CD11b and CD54 were obviously decreased, those of CD4 and CD8 were restored to a certain degree, and the ratio of CD4 +/CD8 + was relatively increased. The results suggest that the effects of XI for relieving cerebral lesions may be related with its decreasing the expressions of CD11b and CD54, increasing the ratio of CD4 +/CD8 +, and restoring the immune function.