单细胞凝胶电泳技术的现场应用及健康人群的表现特征

Application of single cell gel electrophoresis to healthy population and its characteristics

目的介绍一种改良的单细胞凝胶电泳技术同时检测血白细胞ＤＮＡ链断裂和ＤＮＡ－蛋白交联并将其应用于部分健康人群。方法６０名健康人群，每人血样分Ａ、Ｂ两份，Ｂ份在细胞裂解后加蛋白酶Ｋ，其余同常规方法；Ｂ份尾矩为总的ＤＮＡ链断裂量，Ｂ份与Ａ份尾矩的差值为ＤＮＡ－蛋白交联量。结果ＤＮＡ链断裂在性别、年龄和饮酒与否方面的分布无显著性差异，而吸烟可使其显著增加；上述各因素对ＤＮＡ－蛋白交联均无显著影响，后者在该研究人群中的分布里正态（２．４６ ×１０６± ０．７０ ×１０６）。结论该研究为职业接触人群 ＤＮＡ损伤的监测提供了方法学和对照数据的参考。

Objective To introduce a modified single cell gel electrophoresis for detecting DNA strand breaks (DSB) and DNA-protein crosslinks (DPC) in white blood cells simultaneously, and apply it to healthy population. Methods Each blood sample from 60 healthy people was divided into two shares (A and B), adding proteinase K into B after lysis. The other procedures were the same as ordinary method. Tailmom in B was foe total content of DSB and the different tailmom between B and A was that of DPC. Results it seemed to have no significant differences in DSB among sex, age and alcohol drinking, but it did due to smoking. All the factors have no marked influence on DPC in healthy population as it was in normal distribution (2 .46 ×106± 0 .70 ×106). Conclusion This study offered references in methodology and control data for monitoring DNA damage in occupational population.