阿魏酸促进黄芪甲苷在大鼠十二指肠吸收作用的研究

Enhancing effects of Ferulic acid on duodenum absorption of Astragaloside Ⅳ in rats

目的:研究当归主要成分阿魏酸对黄芪提取物中黄芪甲苷的肠道吸收影响,初步探讨当归黄芪药对配伍机制。方法:采用大鼠在体单向肠灌流模型研究不同浓度黄芪甲苷在十二指肠的吸收特性和阿魏酸对黄芪甲苷的促吸收作用。结果:根据大鼠外翻肠囊模型研究结果,得知黄芩甲苷吸收顺序为:十二指肠>空肠>结肠>回肠,阿魏酸对十二指肠的促吸收效果最显著,因此选择十二指肠作为研究肠段;在体单向灌流法结果显示,黄芪甲苷在十二指肠的表观通透系数(Papp)和吸收速度常数(Ka)与浓度有关,且阿魏酸对各浓度的黄芪甲苷皆有促进作用(P<0.05)。结论:十二指肠是黄芪甲苷的主要吸收部位,当归主要成分阿魏酸对黄芪提取物中黄芪甲苷十二指肠吸收具有促进作用。

Objective： To explore the intestinal absorption mechanism of astragalus extract and the enhancing effects of Ferulic acid on intestinal absorption of Astragaloside Ⅳ. Methods： In situ single pass intestinal perfusion model was performed to study the absorption of various concentrations of Astragaloside Ⅳ and the effects of Ferulic acid on its absorption. Results： According to the isolated everted intestine model, the absorption sequence of Astragaloside Ⅳ in different rat intestines was as follows： duodenum〉jejunum〉ileum〉colon; Apparent permeability values （Papp） and absorption rate constant （Ka） of Astragaloside Ⅳ in duodenum varied with the concentrations. And Ferulic acid promoted the absorption of Astragaloside Ⅳregardless of the concentration of Astragaloside Ⅳ （P〈0.05）. Conclusion： Duodenum is the main absorption site of Astragaloside Ⅳ. Ferulic acid promots the absorption of Astragaloside Ⅳ.