细胞自噬在吉非替尼治疗非小细胞肺癌中的作用及其机制研究

The Role of Geftinat in Non Small Cell Lung Cancer Induced by Autophagy

目的:观察吉非替尼对非小细胞肺癌细胞自噬的影响,并探讨其机制。方法:将BalB/CA-nu品系裸小鼠分成两组,各组20只,均造肺癌模型,其中吉非替尼治疗组小鼠在肺癌组模型的基础上给予吉非替尼25mg/kg 14d,停药后剖杀。取组织切片,通过免疫荧光、RT-PCR以及Western blot的试验方法检测自噬相关基因Beclin1和MAPLC3的表达。结果:免疫荧光、RT-PCR以及Western blot的试验方法检测均发现肺癌组小鼠组织中Beclin1和MAPLC3表达较低,而在吉非替尼处理组中,Beclin1和MAPLC3的表达明显升高,差异有统计学意义。结论:吉非替尼可以通过增强细胞自噬从而发挥对非小细胞肺癌的抑制作用。

Objective： To observe The role of Geflinat in non small cell lung cancer induced by autophagy and to investigate the mechanism. Methods： 40 nude mice were randomly divided into two groups, the group with geftinat was treated by infusing the stomach with the medicine, 25mg/kg. The expression of Beclinl and MAPLC3 were determined by immunofluorescence, RT-PCR and Western blot. Results： The Mma and protein level of Beclinl and MAPLC3 in the group with geftinat treating were both significant higher by immunofluorescence, RT-PCR and Western blot. Conclusion： Geftinat can inhibit the progress of non small cell lung cancer by influence autophagy.