摘要
目的 研究新生大鼠缺氧缺血性脑损伤 (HIE)时脑组织单核细胞趋化蛋白 1(MCP 1)蛋白在缺氧缺血后不同时期的表达和单核细胞 /小胶质细胞的浸润情况。方法 2 4只新生大鼠按缺氧缺血后不同生存时点分组 ,免疫组化法检查脑组织MCP 1蛋白的表达 ,光镜下观察脑组织切片HE染色下单核巨噬细胞浸润和神经元变化情况。结果 新生大鼠缺氧缺血后MCP 1蛋白开始表达 ,2 4～ 48小时达到高峰 ,72小时仍有表达。单核细胞 /小胶质细胞的浸润在缺氧缺血后逐渐增多 ,2 4小时达到高峰。结论 新生大鼠缺氧缺血后损伤部位脑组织中单核巨噬细胞浸润增加与损伤部位MCP 1蛋白始表达增加有关。
Objective To investigate the correlation between the expression of Monocyte chemoattractant protein 1 (MCP 1) protein and monocyte/microglial infiltration in brain tissues of hypoxic ischemic neonatal rats.Methods Immunohistochemical and histological techniques were used to evaluate the expression of MCP 1 protein and monocyte/microglial infiltration and the damage of neuron in hypoxic ischemic brain tissues at different time after hypoxia ischemia.Results There wasa significant increase in MCP 1 protein expression in the damage brain tissues that peaked from 24h to 48h after hypoxia ischemia;and the number of monocyte/microglial infiltration was also increasing that peaked at 24h after hypoxia ischemia.Conclusion These data indicate that the expression of MCP 1 protein was significantly correlated with the degree of monocyte/microglial infiltrtion in hypoxic ischemic brain tissue.
出处
《江苏医药》
CAS
CSCD
北大核心
2001年第2期99-100,共2页
Jiangsu Medical Journal
