新橙皮苷二氢查尔酮对流感病毒诱导小鼠肺气血屏障损伤的保护作用

Protective Effect of Neosperidin dihydrochalcone on Influenza Virus-induced Air-blood Barrier Injury in Mice

目的 探讨新橙皮苷二氢查尔酮（NHDC）对流感病毒诱导小鼠肺气血屏障损伤的保护作用.方法 建立流感病毒感染小鼠肺炎模型,给予NHDC灌胃治疗.计算小鼠肺指数、湿干比值、肺组织切片HE染色观察其病理形态变化,ELISA法检测感染后第6天血清中IL-6、LTB4和ICAM-1的含量.免疫组化法检测肺组织水通道蛋白5（AQP5）蛋白表达水平.结果 流感病毒感染小鼠肺指数和湿干比值明显升高、肺组织间质炎性细胞渗出,以中性粒细胞浸润为主,伴明显的肺泡充血、水肿,肺组织AQP5蛋白表达水平明显下降;而NHDC可明显逆转上述病理症状.结论 NHDC对流感病毒诱导的小鼠肺组织气血屏障损伤有明显的保护作用,其可能是通过上调AQP5蛋白表达而发挥作用.

Objective To explore protective effects of neosperidin dihydrochalcone （NHDC） on air-blood barrier injury in influenza virus infected mouse model. Methods The pneumonia model was established by infection of influenza virus. Model mice were treated with NHDC at different dose by gavage. The pulmonary index, wet/dry mass （W/D） ratio and the pathological changes of lung tissue stained with HE were investigated The levels of serum IL-6, LTB4 and ICAM-1 were determined by ELISA, while expression of aquaporin 5 （AQP5） was detected by immunohistochemistry. Results After challenge, the infected mice had obvious increase in pulmonary index and W/D ratio, which manifested by accumlation of significant numbers of neutrophils, accompanied by marked pulmonary edema and high levels of serum IL-6, LTB4 and ICAM-1. The expression of AQP5 was also significantly increased in the lung of infected mice. However, oral administration of NHDC significantly reversed those adverse factors. Conclusion Influenza virus could cause air-blood barrier damage in the lung of infected mice, and it may be related to the expression ofAQP5. NHDC shows obvious effect to the injury.