单羧酸转运蛋白1、2在人星形胶质细胞瘤中的表达变化及其与病理级别之间的关系

Expression of monocarboxylate transporter 1 and 2 in human astrocytoma： A correlation with pathological grades

目的：观察单羧酸转运蛋白（MCT）1、2在人不同病理级别星形细胞瘤组织中的表达差异，探讨星形细胞瘤增殖、生长的分子机制。方法：收集人不同病理级别星形细胞瘤标本51例，运用H-E染色作病理诊断分级，石蜡切片免疫组织化学显色、免疫印迹观察MCTl、2的表达变化。结果：与正常脑组织相比，MCTl、2在人星形细胞瘤中均有高表达l在高级别肿瘤组织中其阳性表达强度明显高于低级别肿瘤组织，并且随病理级别的升高其表达逐渐增强。结论：MCTl、2在人星形细胞瘤组织中表达增强，其强度变化与肿瘤的恶性程度呈正相关。

Objective： To investigate the expression patterns of monocarboxylate transporter MCT1 and MCT2 in human astrocytoma with different pathologic grades, and to explore the molecular mechanism underlying the growth and proliferation of astrocytoma. Methods： Fifty-one astrocytoma samples of different pathological grades were obtained, the relative normal brain tissues around the tumor served as controls. The expression variations of MCT1 and MCT2 at protein levels in human astroeytoma tissues were observed by the techniques of HE staining, immunohistochemisty and Western biotting. Results： Compared with the normal brain tissues, both MCT1 and MCT2 had a strong expression in human astrocytoma, and were enhanced with the increasing of pathologic grades. The expression of MCT1 and MCT2 in high grade astrocytoma was much higher than that in low grade astrocytoma. Conclusion： Protein expressions of MCT1 and MCT2 are increased in human astrocytoma compared to normal controls, and have a positive correlation with pathologic grades.