IFN-γ对牙龈卟啉单胞菌内毒素诱导的细胞耐受的影响

Effects of IFN-γon Endotoxin Tolerance Induced by Porphyromonas Gingivalis Lipopolysaccharides in THP-1 Cells

目的:观察IFN-γ对牙龈卟啉单胞菌(porphyromonas gingivalis,P.gingivalis)脂多糖(lipopolysaccharides,LPS)诱导人单核细胞株THP-1细胞耐受后,炎症因子IL-1β和趋化因子IL-8分泌水平的影响。方法:将THP-1细胞用1mg/L IFN-γ预先处理24h,同时采用1mg/L P.gingivalis LPS刺激该细胞24h,洗脱后,采用P.gingivalis LPS再刺激24h,构建内毒素耐受模型。采用大肠杆菌(escherichia coli,E.coli)LPS作为阳性对照。运用ELISA技术检测细胞条件培养液中IL-1β和IL-8分泌水平的变化。结果:P.gingivalis LPS重复刺激THP-1细胞后,IL-1β分泌水平较第一次刺激后明显降低(P<0.05),IL-8分泌水平与第一次刺激后差别不大。经过IFN-γ预先处理的细胞,P.gingivalis LPS重复刺激诱导细胞耐受后,IL-1β分泌水平较无预处理的细胞明显增高(P<0.05),IL-8分泌水平无明显变化。结论:IFN-γ能够促进P.gingivalis LPS诱导的耐受细胞分泌IL-1β,进而可能影响牙周组织的炎症和免疫反应。

Objective： To observe the effects of IFN-γ on endotoxin tolerance induced by porphyromonas gingivalis （P. gingivalis） lipopolysaccharides （LPS） in THP--1 cells. Methods： THP--1 cells were cultured with 19g/ ml IFN-γ with or without 1μg/mL P. gingivalis LPS for 24h, washed and then restimulated with the same LPS for additional 24h. Escherichia coli （E. coil） LPS was served as positive control. The levels of IL-1β and IL--8 in supernatants were detected by ELISA. Results： After restimulations for another 24h, IL-1β production was decreased significantly compared with that secreted by the cells stimulated with P. gingivalis LPS only once （P〈0. 05）, while there were no significant differences in the levels of IL-8 secreted by the cells with and without repeated P. gingivalis LPS stimulations. In addition, the levels of IL-1β secreted by the cells with IFN-γ priming were significantly higher than those without IFN-γ pretreatment （P〈0.05）. Conclusion： IFN-γcan restore the production of IL-1β in P. gingivalis LPS tolerized--THP-1 cells, which might have an effect on periodontal inflammation.