热疗联合CTL对裸鼠移植瘤的抑瘤效应研究

Antitumor Effect of Hyperthermia Combined with Immunotherapy of Sensitized CTL on Transplantation Tumor of Nude Mouse

目的:研究热疗(HT)联合CTL(致敏的T淋巴细胞)对裸鼠移植瘤的抑瘤效应。方法:建立50只裸小鼠舌癌Tca8113移植瘤动物模型,随机分为5组(对照组、加热处理组、致敏CTL处理组、加热+致敏CTL处理组、致敏CTL+加热组)。于肿瘤接种后8周处死各组动物,提取肿瘤组织标本,行病理学检查。并采用TUNEL法检测肿瘤组织的凋亡情况,运用流式细胞仪检测肿瘤细胞的凋亡率,采用SABC法检测裸鼠移植瘤HSP70的表达水平。结果:HT+CTL+IL-2组和CTL+IL-2+HT有显著的抑瘤效应,抑瘤率为68.85%和61.48%,且该组的肿瘤细胞凋亡率和HSP70表达OD值与其余对照组比较均有显著性差异(P<0.01)。结论:热疗联合致敏CTL的免疫治疗有显著的抑瘤效应,HSP70-PC(热休克蛋白70免疫复合物)起到了抗原提呈和肿瘤抗原疫苗作用。

Objective.. To explore the antitumor effect of hyperthermia combined with sensitized CTL on transplantation tumor of nude mouse. Methods： Fifty nude mice with transplanted Tca8113 tongue squamous cell carcinoma were established and randomly divided into control , HT, HT＋ CTL＋ IL- 2, CTL＋ IL- 2 ＋ HT and CTL＋ IL-- 2 groups. 8 weeks after tumor transplantation, animals in each group were sacrificed, and the tumor tissue was obtained for pathological examination. Besides, apoptosis of tumor issue and apoptosis rate of tumor cell were respectively assessed by TUNEL method and flow cytometer, and the expression of HSP70 was examined by SABC method. Results：The tumor restrain rate of HT＋ CTL＋ IL-- 2 was 68.85 %. The tumor restrain rate of CTLq- IL- 2 ＋ HT group was 61.48%. Furthermore, there was a significant difference regarding the apoptosis rate of tumor cell and the OD value of HSPT0 among HT-I-CTL＋IL--2 group, CTL-I-IL-2＋ HT group and the other groups （P〈 0.01）. Conclusion.. H yperthermia combined with immunotherapy of sensitized CTL shows a significant antitumor effect. During the process, HSP70--PC plays an important role in linking the tumor antigen peptide with APC as tumor antigen vaccine.