肿瘤坏死因子受体相关蛋白1在上皮性卵巢癌组织中的表达及意义

Expression and significance of tumor necrosis factor receptor associated protein 1 in epithelial ovarian cancer

目的:验证并探讨肿瘤坏死因子受体相关蛋白1(tumor necrosis factor receptor associated protein 1,TRAP1)在上皮性卵巢癌中的表达及临床病理意义。方法:利用免疫组织化学和Real-time RT-PCR方法检测114例上皮性卵巢癌组织、50例卵巢良性肿瘤和38例卵巢正常上皮组织中TRAP1的表达。结果:免疫组织化学染色和RT-PCR结果显示,卵巢癌患者TRAP1蛋白和mRNA表达水平明显升高,与正常对照组、卵巢良性肿瘤组相比差异均有统计学意义(P<0.05),TRAP1蛋白和mRNA表达水平在卵巢癌的不同组织学类型间、不同病理分级间差异有统计学意义(P<0.05),而在不同年龄、不同FIGO分期、有无大网膜转移、有无淋巴结转移及不同腹水量间差异均无统计学意义(P>0.05)。结论:TRAP1在卵巢上皮性癌中呈高表达,提示TRAP1可能与上皮性卵巢癌的发生、发展有关。

Objective：To explore the levels of TRAP1 and its roles in patients with ovarian tumor, and investigate the correlation between the expressions of TRAP1 in ovarian tumor tissues and related clinico- pathological characteristics. Methods： 38 health women, 50 cases of benign ovarian tumors and 114 ca- ses of epithelial ovarian cancers were examined by Real-time RT-PCR and immunohistochemieal staining. Results： The immunohistochemical analysis demonstrated that TRAP1 protein was mainly located in the cytoplasm, the protein and mRNA expression of TRAP1 in ovarian cancer were significantly increased compared with those of normal control and benign tumor （P 〈 0.05 ）. The protein and mRNA expression of TRAP1 was related to histological grade and pathologic types （ P 〈 0.05 ）, but not age, clinical sta- ges, lymphnode metastasis or omental metastasis, and the amount of ascites （ P 〉 0.05 ）. Conclusion： The high expression of TRAP1 may play potential role in epithelial ovarian cancer occurrence and pro- gress.