药物作用下的广州管圆线虫幼虫表皮纹理变化的数字化特征研究

Research on Digital Features of Angiostrongylus Cantonensis Surface Texture Under Medication

【目的】通过采集药物作用下广州管圆线虫3/4期幼虫表面纹理变化的超微结构图像,进行数据化特征的研究,探讨可能的药物作用规律。【方法】本文采用基于灰度共生矩阵的图像纹理分析方法,对体内用药、体外用药和不用药3组共21条的广州管园线虫幼虫表皮电镜图像进行预处理后,分别在不同的扫描方向下提取对比度、能量、相关性、同质性和熵5个特征值,并进行单因素方差分析、秩和检验以及描述性统计分析。【结果】样本图像在4个方向上的几乎所有特征都有统计学意义,其中能量和熵在各方向上均具有显著性差异,对比度和同质性特征值在不同方向角度上存在差异,并呈现一定的分布规律。【结论】虫体纹理的数字特征可用于识别和评估广州管圆线虫的用药状态。

[Objective] To research digital data characteristics of the Guangzhou Angiostrongylus larvae surface texture in 3/4 stage by their uhrastructural images, under the impact of drug influences, then discuss the regularity of this impact. [Method] There are three groups of 21 Angiostrongylus cantonensis （In vivo medication, in vitro medication and without medication）. After preprocessing the samples, the contrast, homogeneity, energy, entropy, and correlation are extracted from them in different scanning angles by using the texture images analyzing method based on the grey level co-occurrence matrix. Then all data of these eigenvalues are analyzed by variance and rank statistical analysis. [ Results ] Almost all features of the sample images in four angles have statistically significant. Especially the energy and the entropy in each direction have significant difference, the contrast and the homogeneity in different angles exist significant disparity and some distribution rules. [ Conclusion ] The digital eigenvalues of textures can be used to distinguish and assessment the medicine effect of A ngiostrongylus cantonensis.