舒芬太尼在兔眼局部用药的急性毒性研究

Ocular acute toxicity of sufentanil in rabbits

目的：研究舒芬太尼对兔眼部用药的急性毒性反应，为其临床局部安全用药提供理论依据。 方法：将16只健康成年新西兰兔随机分成4组。1组为对照组，以空白溶剂作溶媒对照；2，3，4组家兔左眼分别滴舒芬太尼注射液5μg（2滴，5min 内）、7．5μg（3滴，10min内）、10μg（4滴，15min内），同时各组右眼以9g/L NaCl溶液作为自身对照，滴速相同。滴药后的7 d后进行局部毒性研究。 结果：肉眼及裂隙灯观察：各组角膜无混浊、结膜无明显充血和水肿、无分泌物、虹膜正常，无产生暂时闭目现象。内皮照相：各组的角膜内皮细胞计数无统计学差异；光镜观察：结膜、角膜、角巩缘、虹膜、睫状体、视网膜及视神经均未发现有病理形态学损伤。 结论：单次使用5～10μg 舒芬太尼眼部给药进行镇静、镇痛在短期内是安全的。

AIM： To investigate the ocular acute toxicity of sufentanil in rabbits, in order to provide the scientific basis for its safe administration and reasonable combination. METHODS： Sixteen healthy rabbits were randomly divided into 4 groups （groups 1-4）, control （vehicle）, low （5μg, 2 drops in 5min）, moderate （7.5μg, 3 drops in 10min） and high doses （10μg, 4 drops in 15min） of sufentanil-treated groups. Solvent without sufentanil was administered to the left eyes in the vehicle control group （group 1）.Meanwhile, 9g/L NaCl solutions were given to the right eyes in all 4 groups at equal drip rate for auto-control.After 7d, ocular toxicity was firstly evaluated. RESULTS：Observed with naked eye and slit lamp, no changes including corneal opacity, conjunctival congestion, chemosis, eye secretions, iris abnormalities and temporal eye closed was in all groups.With slit-lamp microscope, the counts of corneal endothelium cells in all groups have no statistically significant difference.With light microscope, no pathomorphological injury in the conjunctiva, cornea, corneoscleral junction, iris, ciliary body, retina and optic nerve were found. CONCLUSION： The ophthalmic application of sufentanil alone （5-10μg） for sedation and analgesia is safe in a short period.