蛋白质组学方法在玻璃体研究中的应用

Proteomic analyses in the study of vitreous humour

玻璃体以透明的凝胶状态充填于玻璃体腔内,当视网膜发生病变时,相邻的玻璃体的蛋白质构成会发生一些变化,这在很多研究中得到证实。最近10a,蛋白质组学技术被用来研究玻璃体的蛋白质组,希望发现与糖尿病性视网膜病变(diabetic retinopathy,DR)病因相关的特异蛋白质。最近对动物自身免疫性葡萄膜炎的玻璃体样本进行蛋白质组学研究,发现了与眼内炎症相关的特异蛋白质。

Vitreoushumour （ VH） is a transparent, highly hydrated gel, which occupies the vitreous cavity. The protein composition of the VH would change in pathological conditions of the retina, which has been testified in many studies.In the last decade, proteomics analyses have been performed to study the proteome of the human VH and hope to find some specific proteins in the aetiology of diabeticretinopathy （ DR）.Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified some specific proteins in related entophthalmia.