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茶多酚和维生素C对FeSO_-4半胱氨酸诱发离体人血浆脂质过氧化及CCl_4诱发肝自由基损伤的抑制作用 被引量:10

Inhibitory Effects of Tea Polyphenols and Vitamin C on Lipid Peroxidation Induced by FeSO_4-cysteine in Isolated Human Plasma and Carbon Tetrachloride-induced Liver Free Radical Injury in Mice.
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摘要 目的观察不同剂量的茶多酚 (TP)、维生素C(VitC)对FeSO4 半胱氨酸诱发离体人血浆脂质过氧化及CCl4诱发肝自由基损伤的抑制作用。方法 ( 1 )在FeSO4 半胱氨酸诱发离体人血浆脂质过氧化损伤体系中 ,分别检测 0 .3、0 .9、2 .7、8.1mg/L(C~F组 )的TP及 3、9、2 7、81mg/L(G~J组 )的VitC对该体系过氧化脂质 (MDA)生成的影响 ;( 2 )雄性昆明种小鼠 36只 ,随机分为 4组 (n =9) ,分别为空白对照组 (A)、单纯损伤组 (B)、茶多酚 1 0 0mg/kg组 (C) ,以及VitC 1 0 0mg/kg组 (D)。除空白对照外 ,用药组以相应剂量的防护药物灌胃 1次 /日 ,共 3次。末次灌胃 1 2h后 ,除空白对照外 ,其他 3组均用 2 30mg/kg的CCl4灌胃 ,36h后 ,各组小鼠均断头处死 ,分别检测肝匀浆中MDA的含量。结果TP、VitC均可剂量相关地抑制FeSO4 半胱氨酸诱发的离体人血浆脂质过氧化损伤 ,其中TP抑制率分别为30 .7% ,32 .0 %、46.9%、59.7% ;VitC的抑制率分别为 8.3%、41 .4%、47.7%、52 .7%。在CCl4诱发肝自由基损伤体系中 ,同等剂量的TP、VitC对MDA的抑制率分别为 45.2 %、42 .8%。结论在FeSO4 半胱氨酸诱发的人离体血浆过氧化损伤体系中 ,TP( 0 .3~ 8.1mg/L)及VitC( 9~ 81mg/L)对脂质过氧化均有明显的抑制作用 (P <0 .0 1 ) ;同等剂量下 。 Objective To investigate the inhibitory effects of tea polyphenols(TP) and Vitamin C(Vit C) on FeSO 4 cysteine induced lipid peroxidation in isolated human plasma and carbon tetrachloride(CCl 4) induced liver free radical injury in mice. Method The experiment included two parts:(1) In FeSO 4 cysteine induced lipid peroxidation system of isolated human plasma, malondialdehyde (MDA) content was detected after administration of different concentrations of TP(0.3~8.1 mg/L,as C~F group) and Vit C(3~81 mg/L, as G~J group) respectively;(2) Thirty six male Kunming mice were randomly divided into four groups : control group(A), CCl 4 damage group(B), TP protection group(C) and Vit C protection group(D). TP or Vit C (100 mg/kg) was given orally to group C and D respectively, while the same volume of distilled water was given to the other two groups one time every day, continued for three days. Twelve hours after the final treatment, CCl 4 (230 mg/kg) was given orally to group B ~D . Thirty six hours later, all the mice were decapitated and liver homogenate were prepared for measuring MDA content. Result In FeSO 4 cysteine induced lipid peroxidation in isolated human plasma,the inhibitory rate of TP(0.3~8.1 mg/L) was 30. 7%,32.0%,46.9% and 59.7%,and the inhibitory rate of Vit C(3~81 mg/L) was 8.3%,41.4%,47.7% and 52.7% for various dosages. In the CCl 4 induced liver free radical injury system, the inhibitory rate of the same dosage of TP and Vit C was 45.2%,42.8% respectively. Conclusion TP (0.3~8.1 mg/L) and Vit C (9~81 mg/L) had inhibited lipid peroxidation induced by FeSO 4 cysteine in isolated human plasma significantly, and the inhibitory effects of TP was superior to that of Vit C at the same dosages. The same dosage of TP and Vit C had remarkable inhibitory effects on the CCl 4 induced liver free radical injury,but there was no significant difference between the two groups.
出处 《航天医学与医学工程》 CAS CSCD 北大核心 2001年第1期50-53,共4页 Space Medicine & Medical Engineering
基金 全军医药卫生科研基金资助课题!( 96Q0 2 9)
关键词 茶多酚 维生素C 自由基 四氯化碳 肝损伤 tea polyphenols liver blood plasma vitamin C free radicals Carbon tetrachloride(CCl 4)
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