清脑方对缺血性眩晕大鼠脑损伤的保护作用

Protective effect of the Chinese medicine Qingnaofang on brain injury in ischemic vertigo rats

目的初步研究清脑方(Qingnaofang,QNF)对缺血性眩晕大鼠脑损伤的保护作用及其作用机制。方法采用手术结扎右侧颈总动脉和锁骨下动脉致大鼠右侧半脑不完全脑缺血建立缺血性眩晕大鼠模型。分为模型组,QNF 1.04、0.52、0.26 g/kg组,盐酸地芬尼多15 mg/kg组,银杏叶片5.76 mg/kg组以及假手术组,观察QNF对旋转刺激缺血性眩晕大鼠跳台逃避潜伏期的影响,取材并测定动物缺血侧组织Lac、LDH、SOD、MDA、NO及NOS的含量或活性。结果 (1)与模型组相比,QNF 1.04、0.52、0.26 g/kg组大鼠跳台逃避电击潜伏期分别缩短53.6%(P<0.01)、33.8%(P<0.05)、56.5%(P<0.01)。(2)QNF 1.04、0.52、0.26 g/kg均可显著降低缺血侧脑组织中Lac的含量以及LDH的活力(P<0.05,P<0.01),降低其TNOS及iNOS活力(P<0.01);QNF 0.52 g/kg剂量能够明显降低缺血侧脑组织中SOD活力;QNF 0.52、0.26 g/kg剂量可显著降低其MDA和NO的含量(P<0.05,P<0.01)。结论 QNF对缺血性眩晕大鼠脑损伤有一定的保护作用,能够减轻模型动物的眩晕症状,其脑保护作用机制可能与改善缺血脑组织能量代谢,减少氧化应激和炎性损伤有关。

Objective To explore the therapeutic action and possible mechanism of the Chinese medicine Qingnaofang （QNF） on brain injury in ischemic vertigo rats. Methods 228 Sprague-Dawley rats were used in this study. The rat models of ischemic vertigo were induced by ligation of the right common carotid artery and subclavian artery. The rats were randomly divided into model group, QNF 1.04, 0. 52, 0. 26 g/kg groups, Ginkgo biloba leaves extract tablet 0. 00576 g/kg group and sham group. Escape latency of the rats was recorded to measure the degree of vertigo, and the content or activity of Lac, LDH, SOD, MDA, NO and NOS in the ischemia side brain tissues was determined to evaluate the protective effect of QNF. Results 1. The latency of rats was remarkably decreased after treated with QNF 1.04, 0. 52, andO. 26 g,/kg, when compared with the model rats （53.6% P 〈 0.01, 33.8% P 〈 0.05 and 56.5% P 〈 0.01,respectively）. 2. The Lac content and LDH activity were significantly reduced in the brain tissue of cerebral ischemia rats treated by QNF 1.04, 0. 52, and 0. 26 g/kg for seven days, compared with that of the model group （P 〈 0. 05, P 〈 0.01 ）, as well as TNOS and iNOS activity （P 〈0.01 ）. QNF 0. 52 g/kg remarkably decreased SOD activity in the brain tissue of 7-day cerebral ischemia rats （P 〈0.01 ）. QNF 0. 52 and 0.26 g/kg significantly decreased MDA and NO content in the brain tissue of 7-day cerebral ischemia rats （P 〈 0.01 ）. Conclusions There is a protective effect of QNF on ischemic vertigo rats. This effect may be related with the improvement of energy metabolism and reduction of oxidative stress and inflammatory damages.