兔VX2肝癌模型制作改良及影像学评价

Improvement in the preparation of rabbit models bearing VX2 liver tumor for interventional therapy study and imaging evaluation

目的对兔VX2肝癌模型制作进行改良,以用于介入治疗学研究,同时探讨瘤灶的CT表现及CT在检测瘤灶中的作用。方法将VX2瘤细胞接种于兔皮下使其成瘤并传代;新西兰兔24只,以改良嵌插法建立移植性肝癌模型,于建模后7、14、21 d分别行超声、CT及血管造影检查,用于检测兔肝VX2瘤灶,评估瘤灶生长变化;随后处死动物,进行尸解,评估影像检查结果。结果 24只(100%)动物以改良嵌插法建立移植性肝癌模型全部成功。瘤灶以种植后2周CT显示最清楚和典型,直径1 cm^2 cm左右,平扫呈低密度或等密度,动脉早期明显强化,门脉期呈低密度,与周围肝组织分界较清楚。肝动脉造影显示肿瘤富血供。而种植后超过3周的肿瘤大部分发生坏死。结论嵌插改良法是一种值得推广的建立移植性肝癌模型的方法;在对瘤灶进行影像学评价上应尽量选择CT检查,接种后1周左右的瘤灶较小而难以观察;2周左右呈肝动脉源性血供丰富的约1 cm^2 cm的实体瘤,造影征像为肿瘤血管与肿瘤染色;3周以上瘤灶大多出现明显示坏死;因此对1~2cm大小的兔VX2肝癌瘤体,最适合行血管造影检查。

Objective To improve the preparation of rabbit models bearing VX2 hepatoma for interventional therapy study, and to investigate their CT features. Methods Eighty New Zealand white rabbits were implanted with VX2 hepatoma in the deep aspect of the left lobe of liver. CT scanning was performed in the experimental animals at 1 , 2 and 3 weeks after implantation. The plain, early arterial phase and portal phase images were obtained. Results Twenty-four （100%） rabbits were successfully implanted with VX2 hepatoma in the liver. On CT scans taken at 2 weeks after implantation, the tumors showed most clear and typical images： 1 -2 cm in diameter, hypodensity or isodensity on plain CT scans, markedly enhanced early arterial phase images, hypodensity on portal phase images, well discernible from the surrounding liver tissues. Hepatic angiography showed that the tumor was hypervascular. Necrosis was seen in most animals at 3 weeks after implantation. Conclusions The rabbit model bearing VX2 hepatoma is more suitable for interventional therapy study. CT is very useful in evaluating the tumors.