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甲流病毒NS基因降低H5N1禽流感病毒对小鼠的致病力的研究

The NS gene of the 2009/H1N1 virus attenuated the virulence of H5N1 avian influenza virus
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摘要 为研究NS基因对流感病毒致病力的影响,本实验以一株H5N1禽流感病毒(AIV)A/duck/Guangxi/35/2001(DK/35)为亲本病毒株,利用反向遗传技术,以我国首例甲型H1N1流感病毒分离株A/Sichuan/01/2009(SC/01)的NS基因对DK/35株进行单基因替换,拯救出一株重组H5N1 AIV。动物实验表明,SC/01株的NS基因能够使DK/35株对小鼠的致病力减弱。我们推测SC/01株的NS基因主要是通过调节宿主干扰素的产生致弱DK/35株的毒力。进一步对致弱机制进行系统研究,为生产弱毒活疫苗提供一种新的策略。 To study the role of NS on the pathogenicity of influenza A virus, we generated a reassortant virus containing the NS gene from the first 2009 H1N1 isolate in China, A/Sichuan/01/2009（SC/01）, in the background of a H5N1 avian influenza virus （AIV）, A/duck/Guangxi/35/2001（DK/35）, using reverse genetics. Mouse experiment showed that SC/01 NS gene reduced the virulence of DK/35. We speculated that SC/01 NS gene attenuated the pathogenicity of DK/35 by influencing interferon production. Our data emphasizes the need for further study, which provided a novel strategy and foundation for the generation of live attenuated virus vaccine.
作者 孔晖晖 张乾义 张莹 谷春阳 姜永萍 关云涛 陈化兰
机构地区 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点开放实验室 甘肃农业大学
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2014年第3期243-245,共3页 Chinese Journal of Preventive Veterinary Medicine
基金 973专项2011CD505000
关键词 H5N1禽流感病毒 重组 致病性 NS基因 H5N1 avian influenza virus reassortant pathogenicity NS gene
分类号 S852.65 [农业科学—基础兽医学]
  • 相关文献

参考文献10

  • 1http://www, who. int/influenza/human_animal_inter face/H5N 1 _cu- mulative table archives/en/index.html.
  • 2Yasushi I, Kyoko S, Maki K, et al. /n vitro and in vivo charac- terization of new swine-origin H1N1 influenza viruses [J]. Nature, 2009, 460: 1021-1025.
  • 3Hatta M, Gao Peng, Halfmann P, et al. Molecular basis for high virulence of Hong Kong H5Nlinfluenza A viruses [J]. Science 2001, 293(5536): 1840-1842.
  • 4Li Ze-jun, Chen Hua-lan, Jiao Pei-rong, et al. Molecular basis of replication of duck H5N1 influenza viruses in a mammalian mouse model [J]. J Virol, 2005, 79(18): 12058-12064.
  • 5Jiao Pei-rong, Tian Guo-bin, Deng Guo-hua, et al. A single- amino-acid substitution in the NS1 protein changes the patho- genicity of H5N1 avian influenza viruses in mice [J]. J Virol, 2008, 82(3): 1145-1154.
  • 6Seo S H, Hoffmann E, Webster R G, et al. Lethal H5N1 influenza viruses escape host antiviral cytokine responses [J]. Nat Med, 2002, 8(9): 950-954.
  • 7Li Ze-jun, Jiang Yong-ping, Jiao Pei-rong, et al, The NS1 gene contributes to the virulence of HSN1 avian influenza viruses [J]. J Virol, 2006, 80(22): 11115-11123.
  • 8Zhu Qi-yun, Yang Huan-liang, Chen Wei-ye, et al. A naturally occurring deletion in its NS gene contributes to the attenuation of an H5N1 swine influenza virus in chickens [J]. J Virol, 2007, 82(1): 220-228.
  • 9冯华朋,钟功勋,张月梅,李旭勇,李雁冰,施建忠,陈化兰.H5N1亚型人禽流感病毒A/Anhui/2/2005株反向遗传操作系统的建立[J].中国预防兽医学报,2010,32(2):135-138. 被引量：3
  • 10Zhou Bin, Li Yan, Belser J A, et al. NS-based live attenuated H1N1 pandemic vaccines protect mice and ferrets [J]. Vaccine, 2010, 28(50): 8015-8025.

二级参考文献11

  • 1石火英,卢建红,陈素娟,孙蕾,刘秀梵,刘玉良.利用8质粒系统拯救A/Chicken/Shanghai/F/98(H9N2)株禽流感病毒[J].微生物学报,2005,45(3):373-376. 被引量：10
  • 2李泽君,焦培荣,于康震,陈化兰.H5N1亚型高致病性禽流感病毒A/goose/Guangdong/1/96株反向基因操作系统的建立[J].中国农业科学,2005,38(8):1686-1690. 被引量：8
  • 3Fan S F, Gao Y W, Shinya K, et al .Immunogenicity and protective efficacy of a live attenuated H5N1 vaccine in nonhuman primates [J]. PLoS Pathog, 2009, 5: e1000409.
  • 4Li Z J, Chen H L, Jiao P R, et al. Molecular basis of replication of duck H5N1 influenza viruses in a mammalian mouse model [J]. J Virol, 2005, 79: 12058-12064.
  • 5Claas E C, Osterhaus A D, Van Beek R. Human influenza A H5N1 virus related to a highly pathogenic Avian influenza virus [J]. Lancet, 1998, 351: 472-477.
  • 6Neumann G, Watanabe T, Ito H, et al. Generation of influenza A viruses entirely from cloned cDNAs [J]. Proc Natl Acad Sci USA, 1999, 96: 9345-9350.
  • 7Hoffmann E, Neumann G, Kawaoka Y, et al. A DNA transfection system for generation of influenza A virus from eight plasmids [J]. Proc Natl Acad Sci. USA, 2000, 97:6108-6113.
  • 8Hatta M, Gao P, Halfmann P, et al, Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses [J]. Science, 2001, 293(5536): 1840-1842.
  • 9Salomon R, Franks J, Govorkova E A, et al. The polymerase complex genes contribute to the high virulence of the human H5N1 influenza virus isolate A/Vietnam/1203/04 [J]. J Exp Med, 006, 203: 689-697.
  • 10彭亚平,周红波,李春,金梅林.H1N1亚型猪流感病毒的拯救[J].生物工程学报,2008,24(5):857-861. 被引量：10

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中国预防兽医学报
2014年 第3期

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