IgA肾病患者血清6种常见过敏食物特异性抗体的检测

Detection of serum food specific antibodies of 6 common foods in patients with IgA nephropathy

目的初步探讨6种常见过敏食物的主要过敏蛋白与IgA肾病发病的关系。方法采用ELISA双抗体夹心法检测并比较31例IgA肾病患者及80例健康体检者血清中6种常见过敏食物的主要过敏蛋白特异性的IgA1、IgG及IgE水平,并对所有病人进行食物过敏情况问卷调查,观察多种食物抗体水平均明显升高的患者的临床特征。结果 IgA肾病组患者血清中6种常见过敏食物的主要过敏蛋白特异性的IgA1及IgG水平均显著高于健康对照组(P<0.05)。两组中均未检测到食物特异性的IgE。所有病人均无明确的食物过敏史。多种食物(大于4种)特异性IgG抗体水平均明显升高的患者均有蛋白尿。结论某些食物尤其是高致敏性食物在IgA肾病的发病及进程中可能伴有重要角色。

Objective To explore the relationship between the major allergens of 6 common allergic foods and IgA nephropathy. Methods A sensitive sandwich enzyme-linked immunosorbent assay （ELISA） was used to detect the serum levels of food-specific IgA1, IgG and IgE in 31 patients with IgA nephropathy and 80 healthy volunteers. All the patients were examined for a history of food allergy using a questionnaire. Results Serum levels of IgA1 and IgG against the major allergens of the 6 common allergic foods were significantly higher in patients with IgA nephropathy than in healthy volunteers （P＆lt;0.05）. There was no detectable food-specific IgE antibodies in the two groups. No patients had a clear history of food allergy. All the patients with increased IgG levels specific to 4 or more foods simultaneously had proteinuria. Conclusion Some foods especially the highly allergic ones may participate in the pathogenesis and progression of IgA nephropathy.