酪蛋白磷酸肽-非结晶型磷酸钙溶液预防牙釉质脱矿的实验研究

Effect of casein osphopeptide-amorphous calcium phosphate solution in preventing enamel demineralization

目的观察GC护牙素[酪蛋白磷酸肽一非结晶型磷酸钙(CPP-ACP)溶液]预防脱矿液对牙釉质脱矿的效果。方法选取68个因正畸需要拔除的未患龋的前磨牙,均未见牙体表面缺损,彻底清洗后切割成釉质面,打磨成平滑面,保留釉质窗口面,随机分成4组,分别置于0.2%NaF、2%NaF、CPP-ACP、蒸馏水中浸泡7 d,置于脱矿夜中浸泡7 d。进行扫描电镜照射,观察釉质层变化,同时采用电子探针检测仪检测脱矿后釉质层硬组织钙、磷、镁3种元素的分布状况,并进行统计学分析。结果 0.2%NaF、2%NaF、CPP-ACP溶液组牙釉质脱矿率均低于蒸馏水组,且釉质层中钙、磷的含量明显高于蒸馏水组,而镁元素的含量4组无统计学差异,说明0.2%NaF、2%NaF、CPP-ACP均对预防牙釉质脱矿有一定的保护作用。结论 5%CPP-ACP是一种预防牙釉质脱矿的安全有效的措施,其作用与0.2%NaF组相似。

Objective To evaluate the effects of casein osphopeptide-amorphous calcium phosphate （CPP-ACP） in preventing enamel demineralization. Methods Enamel blocks were prepared from premolars extracted from orthodontic patients. The specimens were treated for 30 min daily for 7 days with one of the following agents：deionized water （negative control）, CPP-ACP paste, and NaF solutions （positive controls） （0.2% and 2%）. After the treatments, the specimens were immersed in a demineralizing solution （pH 4.5） for 7 days. The morphology and depth of the lesion were observed using scanning electron microscopy, and the content of Ca, P, Mg in the demineralized enamel was measured by electron probe micro-analyzer. Results The demineralization rates in all the treatment groups were significantly smaller than those in the control group after 7 days of demineralization. Conclusion The application of CPP-ACP to the enamel surfaces can inhibit enamel demineralization with an equivalent effect to 0.2%NaF.