PRR11在胃癌中的表达及其与预后的关系

Expression of PRR11 in Gastric Cancer and Its Relationship with Prognosis

检测PRR11蛋白在胃癌中的表达，分析其表达异常与胃癌临床指标及预后间的关系。用Western免疫印迹比较胃癌和正常组织中PRR11的表达。构建含167例胃癌的组织芯片，用免疫组化法检测PI汛11蛋白在胃癌组织中的表达，统计学分析其与肿瘤大小、肿瘤侵袭、组织分化、淋巴结转移、TNM分期及胃癌患者总生存期之间的关系。PRR11在胃癌组织中的表达高于癌旁组织，在胃癌中的表达率为50．9％（85／167），而在癌旁黏膜中不表达或微弱表达。PRR11的表达与胃壁侵袭、淋巴结转移、疾病分期和组织分化呈正相关（P〈0．05）。单因素生存分析表明，PRRll蛋白阳性表达患者较阴性患者生存期短（45个月VS81个月，P〈0．001）。多因素生存分析也表明，PRR11蛋白阳性表达患者生存期短于阴性患者（95％CI：0．347～0．865，P=0．01）。高表达PRR11与胃癌的发生、进展及预后密切相关，是判断胃癌患者预后的重要指标之一。

To detect PRR11 expression and determine its clinical significance in gastric cancer, tissue microarray blocks containing tumor specimens obtained from 167 patients with gastric cancer were constructed. Expression of PRR11 in human gastric cancer specimens was analyzed using immunohistochemistry （IHC） and Western blot. PRR11 was overexpressed in human gastric cancers compared with that in adjacent non-cancerous tissues by Western blot. IHC results showed that expression of PRR11 was observed in 85 cases （50.9%） of 167 primary gastric cancers. PRR11 expression was significantly associated with gastric wall invasion, lymph node metastasis, tumor progression and poor differentiation （P〈0.05）. PRR11 expression significantly correlated with poor outcome of patients with gastric cancer in univariate （45 months vs 81 months, P〈0.001） and multivariate analyses （95% CI： 0.347-0.865, P=0.01）. Our results showed that PRR11 expression significantly increased with the progression of human gastric cancer, suggesting the importance of PRR11 as a potential biomarker in cancer diagnosis and treatment.