磷酸二酯酶4抑制剂Hemay005对角质形成细胞炎性因子产生的影响

Effects of Phosphodiesterase 4 Inhibitor Hemay005 on the Expression of Inflammatory Factors in Human Keratinocytes

目的为了探讨磷酸二酯酶4抑制剂Hemay005对体外培养的角质形成细胞株HaCaT细胞炎性因子产生的影响。方法分别用重组人肿瘤坏死因子-α(rhTNF-α)、重组人干扰素-γ(rhIFN-γ)和312 nm窄波UVB诱导,采用MTT法检测Hemay005对HaCaT细胞增殖的影响,用ELISA法检测其对HaCaT细胞白细胞介素(IL)-8、sICAM-1和肿瘤坏死因子(TNF)-α产生的影响。结果 Hemay005对HaCaT细胞增殖及25μg/L rhTNF-α诱导的IL-8产生无明显影响,但能剂量依赖性地抑制1 000 U/mL rhIFN-γ诱导的HaCaT细胞产生sICAM-1和124.2 mJ/cm2窄波UVB照射引起的HaCaT细胞产生TNF-α。结论 Hemay005可通过抑制角质形成细胞炎症因子的表达发挥抗炎作用。

Objective To investigate effects of phosphodiesterase 4 inhibitor Hemay005 on the proliferation of keratinocytes and expression of inflammatory factors. Methods HaCaT keratinoeytes were induced by recombinant human TNF-α （rhTNF-α, recombinant human IFN-γ （rhIFN-γ） and 312 nm UVB, respectively. The proliferation of HaCaT cells were detected by MTF method, the production of IL-8, sICAM-1 and TNF-α in HaCaT cells were determined by ELISA. Results It showed that HemayO05 had no significant influence on the proliferation of HaCaT cells and the production of IL-8 induced by 25 ng/mL rhTNF-α, but it could inhibit the production of sICAM-1 induced by 1000 U/mL rhIFN-γand TNF-α stimulated by 124.2 mJ/cm^2 narrowband UVB in a dose-dependent manner. Conclusion Hemay005 might play the role of antipsoriasis by inhibiting the expression of inflammatory factors.