景宁木兰花粉萌发与贮藏特性研究

Pollen Germination and Storage of Magnolia sinostellata

以景宁木兰的花粉为试材,采用花粉离体培养法,用单因子与正交试验研究不同浓度蔗糖、H3BO3、CaCl2所组成的基本培养基对景宁木兰花粉萌发的影响,同时探讨不同贮藏条件和贮藏时间对花粉活力的影响。结果表明:景宁木兰在30 g·L-1蔗糖+200 mg·L-1H3BO3+200 mg·L-1CaCl2的液体培养基上萌发率最高(74.56%)。低温条件下有利于景宁木兰花粉生活力的保持,在-80℃条件下,花粉生活力下降较慢,并且随着贮藏时间的增加,经过硅胶干燥的花粉的活力明显高于湿润花粉的活力。

The pollens of Magnolia sinostellata were employed as experimental material to study the effects of different concentrations of sucrose, boron, calcium on pollen vitality of M.sinostellata through single factorial experiment and the orthogonal design. Moreover, the effects of storage condition and storage time on pollen germination were studied. The results showed that： the optimum medium was sucrose 30 g·L-1＋H3BO3 200 mg·L-1＋ CaCl2 200 mg·L-1, in which the rate of germination was 74.56%. Low temperature contributes to the maintaining of the vitality of M.sinostellata. Storage under -80℃ condition, the vitality of pollen dropped in a slow rate. As the increase of storage time at low temperature, the vitality of pollen dried by silicone was higher than the moist pollen.