人白细胞介素-38的特性分析及B细胞表位的预测

Characteristic Analysis of Human Interleukin- 38 and Prediction of Its B- Cell Epitope

目的:为进一步了解人白细胞介素(IL)-38蛋白质的特性和B细胞表位,从GenBank中查得其序列,用生物信息学等方法预测人IL-38蛋白质的二级结构等特性,并对其B细胞表位进行预测。方法:应用ProtParam tool、ProtScale、Emini和Kolaskar等程序或方法,对IL-38的组成、理化特性、亲水性、柔韧性和可及性等进行分析,以进一步预测其二级结构和抗原表位。结果:IL-38的二级结构主要由无规则卷曲(44.08%)和β-折叠(39.47%)组成。其B细胞表位可能位于第26-36、45-55、78-98和124-143等氨基酸区段。结论:首次利用生物信息学方法对IL-38基因及其蛋白质结构进行了分析和预测,预测结果将有助于确定IL-38的B细胞表位,为IL-38基因功能的深入研究及研制IL-38单克隆抗体提供了理论依据。

Objective：Based on interleukin （IL） -38 amino acid sequence from GenBank,the secondary structure and B -Cell epitope were predicted by bioinformaties. Method ：The ProtParam tool, ProtSeale, Emini and Kolaskar were applied to analyze human IL- 38 pro- tein＇ s composition, physical and chemical properties, hydrophileity ,flexibility, aeeessibility, and ordered to forecast seeondary structures, B -cell epitopes. Result：The secondary structure of human IL -38 eontented random coil （44. 08% ） and extended strand （39.47%）. It showed that the most possible epitopes of IL - 38 were located in or adjacent to amino acid 26 - 36,45 - 55,78 - 98,124 - 143. Conclusion：The view was the first report to analysis the construction of protein IL - 38 by bioinformaties. These results would be helpful for the estimate of the epitopes, provided references for further research of human IL - 38 protein function and a theory basis for de- veloping monoclonal antibodies against human IL- 38.