Wistar大鼠不同生理时期乳腺淋巴管的研究

STUDY ON LYMPHATICS OF THE MAMMARY GLAND IN WISTAR RATS UNDER DIFFERENT PHYSIOLOGICAL PERIODS

乳腺的淋巴管在不同生理时期的发育资料较少 ,研究旨在对不同发育时期的乳腺淋巴管进行定性定量分析。被分析的结构包括淋巴管内皮的面积 (非核区 )、内皮吞饮小泡的面积、内皮间开放连接与关闭连接的数目等。2 0只Wistar大鼠被分成四组 :正常组、妊娠 7天组、哺乳 7天组和哺乳停止后 7天组 ,每组各 5只。动物经主动脉灌流生理盐水后 ,然后灌注 2 0～ 3 0毫升墨汁以区别血管与淋巴管。乳腺组织标本经处理后 ,应用光镜、透射电镜及图像分析等方法对其进行了有关研究。乳腺的淋巴管可分为三类 :叶间淋巴管、小叶间淋巴管和小叶内淋巴管(仅见于哺乳 7天组 )。淋巴管内皮间开放连接的数目占连接总数的百分比在各组分别为 1 2 .71 % (正常组 )、2 8%(妊娠 7天组 )、3 7.61 % (哺乳 7天组 )和 8.1 5 % (哺乳停止后 7天组 )。淋巴管内皮吞饮小泡的面积与内皮总面积(非核区 )的比在各组分别为 4.2 % (正常组 )、1 .9% (妊娠 7天组 )、0 .9% (哺乳 7天组 )和 2 .7% (哺乳停止后 7天组 )。研究结果显示 :在哺乳 7天组和妊娠 7天组 ,开放连接为淋巴管运输的主要通道 ;而在正常组 ,内皮吞饮小泡在运输过程中起重要作用。

Objective: To qualitatively and quantitatively examine lymphatics of the mammary gland during different physiological periods in Wistar rats. Methods: The structures analyzed included the area of the endothelia(non-nuclear area), area of the endothelial vesicles, the number and types of open and close junctions. Twenty Wistar rats were divided into 4 groups: normal, pregnancy on day 7, lactating on day 7, post-weaning on day 7 groups(n=5). The animals were perfused with 0.9% saline and then with 20～30 ml India ink through the aorta to distinguish blood vessels from lymphatics. The mammary gland tissue was treated with routine LM and TEM procedures. The related research were done by LM, TEM and morphometry. Results: The initial lymphatics of the mammary gland were classified into 3 categories:(1) interlobar lymphatic, (2) interlobular lymphatic and (3) intralobular lymphatic (only found in lactating group). The ratio between the open and the total junctions of the lymphatics was 12.71%(normal), 28% (pregnancy on day 7), 37.61% (lactating on day 7), and 8.15% (post-weaning on day 7). The area ratio between the endothelial vesicles and the total endothelia in each group was 4.2%(normal), 1.9% (pregnancy on day 7), 0.9% (lactating on day 7), and 2.7%(post-weaning on day 7). Conclusion: During lactating and pregnancy on day 7, open junctions are the major route for the transport; while in the normal group, the endothelial vesicular transport plays an important role.