血管钠肽抑制低氧刺激心成纤维细胞c-fos的表达

Vasonatrin peptide attenuates hypoxia-induced expression of c Fos gene in cultured cardiac fibroblasts

目的 研究血管钠肽 (VNP)对低氧作用时心成纤维细胞增殖的影响 ,并对其机制进行探讨 .方法 分离纯化乳鼠心成纤维细胞 ,随机分为 4组 :对照组、低氧组 (2 0～ 30m L· L- 1 )、VNP组 (10 - 8～ 10 - 6 mol· L- 1 )和 VNP (10 - 6mol· L- 1 ) +低氧组 .以免疫组织化学染色方法观察各组 c-Fos的表达情况 ,采用激光共聚焦方法测定了 [Ca2 + ]i 水平 .结果 1低氧组较对照组可以显著升高 Fos样免疫阳性细胞数及染色强度 (P<0 .0 5 ) ;2 VNP预处理心成纤维细胞可以减弱低氧的作用 (P<0 .0 5 vs低氧组 ) ,但 Fos的表达量仍高于对照组 (P<0 .0 5 ) ;3对照组 [Ca2 + ]i 水平无显著变化 ,低氧组 [Ca2 + ]i 水平显著升高 ,而 VNP能使升高的 [Ca2 + ]i 水平较对照组和低氧组显著降低 (P<0 .0 5 ) .结论 VNP能减弱低氧对心成纤维细胞生长的刺激作用 ,其机制可能与 Ca2 + 等信号转导分子及 c-

Chief aim of the present work was to investigate vasonatrin peptide (VNP) attenuates hypoxia induced expression of c fos gene in cardiac fibroblasts from cultured neonatal rats. The cultured cardiac fibroblasts were divided randomly into four groups: Control group, hyoxia group (20~30 mL·L -1 ), VNP group (10 -8 ~10 -6 mol·L -1 ) and VNP (10 -6 mol·L -1 )+hypoxia group. The c Fos expression of cardiac fibroblasts was measured by the means of immunocytochemistry, [Ca 2+ ] i concentration was measured by the means of interactive laser cytometer. The studies performed with these techniques show that hypoxia increased significantly the positive cells and mean grey of cardiac fibroblasts ( P <0 05 vs control group), VNP decreased the positive cells ( P <0.05 vs control group) and decreased the level of [Ca 2+ ] i in VNP+hypoxia group. These findings indicate that VNP can attenuate hypoxia induced expression of c fos gene in cardiac fibroblasts which was associated with the changes of the[Ca 2+ ] i.