高浓度氧抑制兔动脉平滑肌细胞的增殖

High oxygen percent acts on the proliferation of aortic smooth muscle cells of rabbits

目的 探讨缺氧和高氧环境对兔主动脉平滑肌细胞增殖的抑制作用 ,间接判断缺氧与冠脉再狭窄的关系和研究氧疗是否能用于 PTCA支架后冠脉再狭窄的防治 .方法 用含 10 0 m L· L- 1小牛血清的 RPMI16 40培养液体外培养新西兰白兔的主动脉平滑肌细胞至 3～ 7代 ,将含 5 0 m L· L- 1小牛血清的 RPMI16 40培养液与动脉平滑肌细胞的混悬液10 0 μL 加入 96孔培养板中培养 ,按照实验分组分别在 10 0 ,2 10 ,2 5 0 ,5 0 0和 75 0 m L· L- 1 O2 及纯氧环境中培养主动脉平滑肌细胞 48h.用 MTT法和 3H- Td R掺入法检测动脉平滑肌细胞增殖的数量 .结果 以 2 10 m L· L- 1 O2 环境下动脉平滑肌细胞增殖的数量为基准对照组 ,其他各组为实验组 .5 0 0 m L· L- 1 O2 至纯氧环境下动脉平滑肌细胞的增殖显著低于对照组 (P<0 .0 5 ) ,2 5 0 m L· L- 1 O2 与 2 10 m L·L- 1 O2环境下主动脉平滑肌细胞的增殖无显著差异 (P>0 .0 5 ) ,10 0m L· L- 1 O2 环境下动脉平滑肌细胞的增殖显著高于对照组(P<0 .0 5 ) .结论 动脉平滑肌细胞的增殖随着培养环境中氧浓度升高而降低 ,缺氧促进动脉平滑肌细胞的增殖 ,高氧对动脉平滑肌细胞增殖具有显著的抑制作用 ;氧疗可能有助于降低冠脉再狭窄率 ,并为高压氧治疗其他以动脉血管?

AIM To investigate the effect of different oxygen percent on the proliferation of aortic smooth muscle cells (ASMCs), indirectly assess the relation between the anoxia and coronary restenosis and observe if oxygen therapy can be used to treat the coronary restenosis after percutaneous transluminal coronary angioplasty (PTCA) and in stent. METHODS New Zealand rabbit ASMCs were cultured in vitro with RPMI 1640 including 100 mL·L -1 calf plasma. 100 μl mixture of ASMCs and RPMI 1640 including 50 mL·L -1 calf plasma was put in every cavity of 96 hole plate to culture for 24 h. Then ASMCs were cultured for 48 h in different mixture that consists of oxygen and N 2 according to experimental groups (100 mL·L -1 O 2, 210 mL·L -1 O 2, 250 mL·L -1 O 2,500 mL·L -1 O 2,750 mL·L -1 O 2, 1000 mL·L -1 O 2). MTT method and 3H TdR incorporation monitored the numbers of ASMCs proliferation. RESULTS Number of ASMCs proliferation in 500 mL·L -1 O 2,750 mL·L -1 O 2, 1000 mL·L -1 O 2 groups were significantly lower than that in 210 mL·L -1 O 2 group ( P <0.05), higher in 100 mL·L -1 O 2 group ( P <0.05), but similar in 250 mL·L -1 O 2 group ( P >0.05). CONCLUSION The oxygen therapy could be used to prevent and treat the coronary restenosis after PTCA and in stent.