γ射线照射人肝细胞对胰岛素样生长因子相关基因表达的影响

Study of γ-ray Irradiation with Expressive Influence of IGF Related Genes in Human Hepatocytes

利用实时荧光定量PCR技术对人肝细胞株受不同剂量(0.1、0.2、0.5、1.0、2.0、4.0 Gy)60Coγ射线照射后不同时间点(6、12、24 h)的胰岛素样生长因子相关基因进行剂量和时间效应关系研究。结果表明,胰岛素样生长因子1受体(IGF1R)在不同剂量及照后不同时间点PCR扩增结果均表现为低表达趋势;胰岛素样生长因子结合蛋白1(IGFBP1)在不同剂量照后6 h点PCR扩增结果均表现为高表达趋势,在照后12 h点均表现为低表达趋势,而在照后24 h点除个别剂量点表现为高表达趋势(0.1 Gy和0.2Gy点),其余剂量点均表现为低表达趋势;胰岛素样生长因子2结合蛋白3(IGF2BP3)除个别剂量点和时间点PCR扩增结果表现为低表达趋势(0.2 Gy照后6、12、24 h及0.5 Gy照后6 h),其余各剂量点和时间点均表现为高表达趋势;胰岛素样生长因子结合蛋白4(IGFBP4)在不同剂量照后6 h点PCR扩增结果均表现为低表达趋势,而在照后12、24 h点则表现为高表达趋势。

The study analyzed the effect-relationship 0.5, 1, 2 and 4 Gy） and different times（6, 12 and of γ-ray irradiation with different doses （0.1, 0.2, 24 h） of IGF related genes in human hepatocytes by real-time fluorescent quantitative PCR. The results indicated that insulin-like growth factor - 1 receptor （IGF1R） showed low expression with different dosage and duration; insulin-like growth factor binding protein - 1 （ IGFBP1 ） showed high expression after radiation 6 h, 24 h showed low expression, 0.1 and 0.2 Gy showed high expression and 0.5, 1, 2 and 4 Gy low expression after radiation 12 h; IGF2BP3 showed low expression with 0.2 Gy and 6 h of 0.5 Gy, and high expression with other dosage and duration; IGFBP4 showed low expression with 6 h, and high expression with 12 and 24 h.