亲代母体长期铅暴露对雄性子代断乳大鼠睾丸中谷胱甘肽的影响

Effects of maternal long-term lead exposure on glutathione in testes of male weaning offspring in rats

目的探讨亲代母体长期铅暴露对雄性子代断乳大鼠睾丸中谷胱甘肽的影响。方法将24只健康SPF级sD雌性大鼠随机分作3组，分别为对照（去离子水）组和0．8、1．5g／L乙酸铅染毒组，每组8只。于妊娠前10d和妊娠期[（20＋1）d]与哺乳期（出生后0～21d）采用自南饮水方式进行染毒。待断乳时，每窝随机抽取l～2只雄性子代大鼠，每组15只，检测血铅含量、睾丸内还原型谷胱甘肽（GSH）与氧化型谷胱甘肽（GSSG）的含量。结果与对照组比较，1．5g／L乙酸铅染毒组雄性仔鼠睾丸内GSH含量以及0．8、1．5g／L乙酸铅染毒组雄性仔鼠睾丸内GSH／GSSG值均较低，差异有统计学意义（P〈0．05）。1．5g／L乙酸铅染毒组雄性仔鼠睾丸内GSH／GSSG值低于0．8g／L乙酸铅染毒组，差异有统计学意义（P〈0．05）。且随着乙酸铅染毒浓度的升高，雄性仔鼠睾丸内GSH含量、GSH／GSSG值均呈下降趋势，而血铅含量和GSSG含量呈上升趋势。结论亲代母体长期铅暴露能对雄性子代断乳大鼠睾丸中谷胱甘肽产生一定影响。

Objective To explore the effects of maternal long-term lead exposure on glutathione in the testes of male weaning offspring of rats. Methods A total of 24 healthy SPF female SD rats were randomly divided into three groups, eight in each, which were treated with lead acetate through drinking water at the doses of 0,0.8 and 1.5 g/L from ten clays before gestation to weaning. Then one or two male offspring rats were selected randomly from each maternal parent at the end of experiment, in total, fifteen male offspring rats in every group were chosen to detect. Atomic absorption spectrometry,HE staining,enzyme linked immunosorbent assay were used to measure Pb2＋ concentrations,possible pathological changes,GSSG and GSH contents. Results Compared with the control group,GSH of testes in the 1.5 g/L lead acetate group and GSH/GSSG of the two lead- treated groups were significantly declined （P〈0.05）; GSH/GSSG of testes in the 1.5 g/L lead acetate group was significantly decreased compared with those in 0.8 g/L lead acetate group （P〈0.05）. As the increase of lead exposure close of maternal parent,GSH and GSH/GSSG of testes showed a downward trend,but Pb2＋ content of blood and GSSG showed an upward trend among male offspring. Conclusion Long-term lead exposure of maternal parent may have effect on glutathione in the testes of male offspring of rats.