常见恶性肿瘤端粒酶活性检测

Telomerase activity of common malignant tumors

目的 探讨端粒酶活性检测在常见恶性肿瘤诊断中的作用。 方法 采用改良的银染端粒重复序列扩增方法 ,对 2 83例恶性肿瘤、6 1例良性肿瘤和 42例癌旁组织进行端粒酶活性检测。结果 2 83例恶性肿瘤中 ,2 6 4例检测到端粒酶活性 ,阳性率为 93 3 % ;6 1例良性肿瘤中 ,4例检测到端粒酶活性 ,阳性率为 6 6 % ;42例癌旁组织中 ,11例检测到端粒酶活性 ,阳性率为 2 6 2 %。结论端粒酶的激活与恶性肿瘤的发生发展密切相关。端粒酶可以作为一种有效的恶性肿瘤标记物用于恶性肿瘤的诊断。

Objective To study the usefulness of detection of telomerase activity in the diagnosis of malignant tumors. [WT5”HZ]Methods Modified telomeric repeat amplification protocol (TRAP) was employed to examine telomerase activity in 283 malignant tumor tissuses, 42 adjacent tissuses and 61 benign tumor tissuses. [WT5”HZ]Results Of the 283 malignant tumors and 42 adjacent tissues, telomerase was positive in 264 (93.3%) and 11 (26.1%), respectively. Only 4 of the 62 (6.6%) benign tumors were telomerase positive. Conclusion Telomerase activity may be used as tumor marker in the diagnosis of malignant tumors.