附子干姜合用丙磺舒、兰索拉唑对NOS抑制型高血压模型小鼠影响观察

The Effects and Interactions of Radix Aconiti Lateralis Preparata Combined with Rhizome Zingiberis and Probenecid,Lansoprazole on NOS Inhibitied Mice

目的:研究附子干姜合用丙磺舒、兰索拉唑对一氧化氮合酶(NOS)抑制型高血压模型小鼠的影响,并初步探讨其作用机制。方法:将雄性ICR小鼠随机分为空白对照组(C组)、左旋N-硝基精氨酸(L-NNA)模型组(M组)、L-NNA+附子干姜组(FG组)、L-NNA+丙磺舒组(B组)、L-NNA+兰索拉唑组(L组)、L-NNA+附子干姜+丙磺舒组(FG+B组)、L-NNA+兰索拉唑+丙磺舒组(L+B组)、LNNA+附子干姜+兰索拉唑+丙磺舒组(FG+L+B组)。各组分别给予0.5%羧甲基纤维素钠(CMCNa)溶液、L-NNA、L-NNA+附子干姜水煎液3g/kg、L-NNA+丙磺舒360mg/kg、L-NNA+兰索拉唑30mg/kg、L-NNA+附子干姜水煎液3g/kg+丙磺舒360mg/kg、L-NNA+兰索拉唑组30mg/kg+丙磺舒组360mg/kg,L-NNA+附子干姜水煎液3g/kg+丙磺舒360mg/kg+兰索拉唑30mg/kg。持续灌胃给药10天后测定各组小鼠的血压,检测血清、心、肝、肾和胃组织中的一氧化氮(NO)和丙二醛(MDA)含量。结果:与C组相比,M组、B组、L组和L+B组血压显著升高(P<0.01,P<0.05,P<0.05),M组和B组大鼠的血清和心、肝、肾、胃中的NO含量下降,MDA含量升高(P<0.01或P<0.05)。与模型组相比,FG组、FG+B组、FG+B+L组小鼠的血压和生化指标均有所改善(P<0.01或P<0.05),其中FG+B组死亡率最低,FG+B+L组其次。结论:FG组、FG+B组、FG+B+L组药物均能升高NOS抑制型高血压小鼠的NO含量,降低MDA含量,从而产生降压、降低组织氧化损伤的作用,其中FG+B+L组小鼠更趋近于正常水平。

Objective：To study the effects and interactions of Radix Aconiti Lateralis Preparata combined with Rhizoma Zingiberis and probenecid, lansoprazole on nitric oxide synthase （NOS） inhibited mice. Methods：Male ICR mice were randomly divided into control group, model group, Radix Aconiti Lateralis Preparata combined with Rhizoma Zingiberis group （FG）, probenecid group （B）, lansoprazole group （L） , FG plus probenecid group （FG-bB）, lansoprazole plus probenecid group （L＋B） , FG plus probenecid and lansoprazole group （FG＋L＋ B）. The mice were intragastricly administrated with CMC-Na, L-NNA, L-NNA-FFG, L-NNA＋ B, L-NNA＋L, L-NNA ＋FG＋B, L-NNA＋L＋B, L NNA＋FG＋L＋B, respectively for continuous 10 days. The changes of blood pressure were measured, as well as the contents of NO and MDA in serum, heart, liver, kidney and stomach. Results：Compared with control group, the blood pressure of M group, B group, L group and L＋B group were increased significantly （P〈（0.01, P〈0.05, P〈0.05）. The contents of NO in serum, heart, liver, kidney and stomach of M group and B group were sharply in- creased, and MDA decreased （P〈0.01or P〈0.05）. Compared with the model group, FG group, FGq-B group and FG＋L＋B group were notably improved in blood pressure and biochemical indicators （P〈0.01or P〈0.05）. The lowest mortality was among the mice in FG-LB group, and then the FG＋L---B group. Conclusion： The FG group, FG＋B group, and FG＋B＋L group respectively posed significant effect on the blood pressure, the contents of NO and MDA to protect the tissues from oxidative impairment, among which the mice in FG＋B-FL group were closer to normal level.