摘要
目的:以不同产地的余甘子酚酸类成分为研究对象,建立酚酸类成分的HPLC指纹图谱,为科学评价和有效控制余甘子有效成分质量提供科学依据。方法:采用Agilent C18色谱柱,流动相为0.2%磷酸水(A)-甲醇(B)梯度洗脱,在276nm波长下对7种不同产地的余甘子药材酚酸类成分进行测定,建立余甘子酚酸类成分的高效液相色谱指纹图谱,通过相似度评价软件对其质量和品质进行评价。结果:对7种余甘子酚酸类成分进行测定,建立了余甘子酚酸类成分指纹图谱,并且确定7种不同产地余甘子酚酸类成分有7个共有峰,且7种酚酸类成分的指纹图谱相似度在0.90以上。结论:建立的指纹图谱有较强的针对性,可为余甘子药材有效成分的研究及其质控标准的制定提供科学依据。
Objective：To establish the HPLC fingerprint of phenolic acids of Fructus Phyllanthi from different regions, and provide the scientific foundation for quality evaluation and quality control of the effective ingredient. Methods： The phenol acids composition of Fructus Phyllanthi from 7 different regions was determined by the following condition： Agilent C18 chromatographic column, 0. 2% phosphoric acid water （A） - methanol （B） mobile phase, gradient elution, under the wavelength of 276 nm. The HPLC finger- prints of phenol acids composition of Fructus Phyllanthi were established and the qualities were evaluated through similarity evalua tion software. Results： The HPLC fingerprints of phenol acids composition of Fructus Phyllanthi from 7 different regions were formed. 7 common peaks were found and the similarity of them were more than 0.90. Conclusion ： The fingerprint was specific and the established method can be used for research and quality control of the effective components in Fructus Phyllanthi.
出处
《亚太传统医药》
2014年第4期31-33,共3页
Asia-Pacific Traditional Medicine
