摘要
目的:以不同产地的余甘子酚酸类成分为研究对象,建立酚酸类成分的HPLC指纹图谱,为科学评价和有效控制余甘子有效成分质量提供科学依据。方法:采用Agilent C18色谱柱,流动相为0.2%磷酸水(A)-甲醇(B)梯度洗脱,在276nm波长下对7种不同产地的余甘子药材酚酸类成分进行测定,建立余甘子酚酸类成分的高效液相色谱指纹图谱,通过相似度评价软件对其质量和品质进行评价。结果:对7种余甘子酚酸类成分进行测定,建立了余甘子酚酸类成分指纹图谱,并且确定7种不同产地余甘子酚酸类成分有7个共有峰,且7种酚酸类成分的指纹图谱相似度在0.90以上。结论:建立的指纹图谱有较强的针对性,可为余甘子药材有效成分的研究及其质控标准的制定提供科学依据。
Objective:To establish the HPLC fingerprint of phenolic acids of Fructus Phyllanthi from different regions, and provide the scientific foundation for quality evaluation and quality control of the effective ingredient. Methods: The phenol acids composition of Fructus Phyllanthi from 7 different regions was determined by the following condition: Agilent C18 chromatographic column, 0. 2% phosphoric acid water (A) - methanol (B) mobile phase, gradient elution, under the wavelength of 276 nm. The HPLC finger- prints of phenol acids composition of Fructus Phyllanthi were established and the qualities were evaluated through similarity evalua tion software. Results: The HPLC fingerprints of phenol acids composition of Fructus Phyllanthi from 7 different regions were formed. 7 common peaks were found and the similarity of them were more than 0.90. Conclusion : The fingerprint was specific and the established method can be used for research and quality control of the effective components in Fructus Phyllanthi.
出处
《亚太传统医药》
2014年第4期31-33,共3页
Asia-Pacific Traditional Medicine