异氟醚对肺泡Ⅱ型上皮细胞Na^+-K^+-ATP酶活性的影响

Effects of isoflurane on Na-K-ATPase activity of cultured primary alveolar type Ⅱ cells

目的 通过原代培养的正常及受 (H2 O2 )损伤的肺泡Ⅱ型上皮细胞 ,探讨异氟醚 (Iso)对肺泡Ⅱ型上皮细胞Na+ K+ ATP酶活性的影响。方法 肺泡Ⅱ型上皮细胞分离、纯化、培养 2 4h后 ,分为六组 :对照组 (不加任何药物 ) ,0 2 8mmol/LIso组 ,2 8mmol/LIso组 ,75 μmol/LH2 O2 组 ,75 μmol/LH2 O2 +0 2 8mmol/LIso组和 75 μmol/LH2 O2 +2 8mmol/LIso组 ,继续培养 3h。分别测定肺泡Ⅱ型上皮细胞Na+ K+ ATP酶活性以及培养液中乳酸脱氢酶 (LDH)活性和丙二醛 (MDA)含量。结果 Iso降低正常肺泡Ⅱ型上皮细胞Na+ K+ ATP酶活性并加重H2 O2 引起的肺泡Ⅱ型上皮细胞Na+ K+ ATP酶活性的降低 ;Iso对正常培养的肺泡Ⅱ型上皮细胞培养液中LDH活性和MDA含量无明显影响 ,但 2 8mmol/LIso可使受H2 O2 损伤的肺泡Ⅱ型上皮细胞培养液中LDH活性和MDA含量增加。结论 Iso可以降低肺泡Ⅱ型上皮细胞Na+ K+ ATP酶活性 。

Objective To explore the effect of isoflurane on Na-K-ATPase activity in cultured primary alveolar type Ⅱ(ATⅡ) cells with or without being injured by H 2O 2.Methods ATⅡcells were isolated from adult rat lungs and incubated for 24h and divided into six groups. Group 1 served as control and received no treatment. Group 2 and 3 ATⅡ cells were exposed to 0.28 or 2.8mmol/L isoflurane. In group 4 cells were exposed to 75μmol/L H 2O 2. In group 5 and 6 cells were exposed to both 75μmol/L H 2O 2 + 0.28 or 2.8mmol/L isoflurane. Each group was incubated for another 2h after the addition of isoflurane and /or H 2O 2. The Na-K-ATPase activity of ATⅡcells ,the LDH activity and the MDA concentration of fluid culture medium were measured by biochemical methods.Results Isoflurane markedly decreased Na-K-ATPase activity in normal ATⅡ cells, but aggravated the decrease in Na-K-ATPase activity induced by H 2O 2. Isoflurane had no effect on LHD activity and MDA concentration of fluid culture medium of normal ATⅡ cells ,but significantly increased LHD activity and the MDA concentration of of fluid culture medium of ATⅡ cells injured by H 2O 2.Conclusions Isoflurane can inhibit Na-K-ATPase activity of ATⅡ cells in vitro, and aggravate the damage of ATⅡ cells caused by oxidants.