濒危药用植物八角莲内生真菌分离鉴定及抗菌活性研究

Isolation,Identification and Antimicrobial Activities of Endophytic Fungi of Dysosma versipellis( Hance) M. Cheng

目的考察广西八角莲[Dysosma versipellis(Hance)M.Cheng]内生真菌的多样性并筛选具有抗菌活性的菌株。方法采用琼脂块法筛选具有抗菌活性的内生真菌;应用ITS-rDNA法鉴定所有分离的内生真菌,并对具有强抗菌活性的菌株进行形态学观察。结果从八角莲根茎叶中共获得19株真菌,其中根12株,叶5株,茎2株;鉴定结果表明,这19株真菌分属于8目13属。抗菌活性筛选发现DV04对金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)和白色念珠菌(Candida albicans)均有较强的抗菌活性,鉴定结果显示,该菌株属于Leptodontidium属暗色有隔真菌(DSE)真菌。结论八角莲中存在丰富的内生真菌,DV04菌株可作为抗菌活性候选菌株进行深入研究。

OBJECTIVE To examine the diversity and antimicrobial activities of the endophytic fungi of Dysosma versipeUis （Hance） M. Cheng. METHODS The identification of the fungi was carried out by rDNA-ITS sequence analysis. The antimicrobial activities of the isolated endophytic fungi were determined by agar block test. RESULTS Nineteen strains of various endophytes were obtained from the roots, stems and leaves of D. versipellis, which were molecularly identified to belong to eight calalogues and thirteen genuses. The DV04 had remarkable antimicrobial activities to Staphylococcus aureus, Escherichia coli, and Candida albicans. Morpho- logical and molecular identification demonstrated that DV04 represented Leptodontidium sp.. CONCLUSION The endophytic fungi in Dysosma versipellis axe diverse and rich. Some strains such as DV04 deserve fmther research as an alternative antimicrobial resource.