亚甲基四氢叶酸还原酶基因多态性与2型糖尿病的相关性

The related study between methylenetetrahydrofolate reductase gene polymorphism and type 2 diabetes mellitus

目的:探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T和A1298C多态性与2型糖尿病(T2DM)的关系。方法:选取106例T2DM患者(病例组)和109名健康对照个体(对照组),采用聚合酶链反应-限制性片段多态性分别检测C677T和A1298C多态位点等位基因频率和基因型并统计分析。结果:2组MTHFR基因C677T和A1298C位点基因型和等位基因频率比较差异均无统计学意义(P>0.05),T2DM发病中2个突变位点之间没有协同作用(P>0.05)。结论:MTHFR基因C677T和A1298C多态位点与T2DM的易感性无明显的相关性。

Objective：To examine the relationship between methyienetetrahydrofolate reductase （MTHFR） gene C677T and A1298C polymorphisms and type 2 diabetes mellitus（T2DM）. Methods：MTHFR gene C677T and A1298C polymorphic sites were assayed in 106 T2DM and 109 healthy subjects by polymerase chain reaction-restriction fragment length polymorphism, and their allele frequency and genotype were statistically analyzed. Results： There was no statistical significance in allele frequency and genotype of MTHFR gene C677T and A1298C between the patients and healthy subjects（ P 〉 0.05 ）, and no synergistic reaction between the MTHFR C677T and A1298C polymorphisms on the risk of T2DM（P 〉0.05）. Conclusions： MTHFR gene C677T and A1298C polymorphism is not a risk factor for patients with type 2 diabetes.