摘要
目的建立灯盏花乙素中5种有机溶剂残留量的分离测定方法。方法采用顶空进样毛细管气相色谱法,色谱柱为DB一1301毛细管柱(30m::〈0.32mmx1.0μm),以N:为载气,FID检测器,程序升温,N,N-二甲基乙酰胺(DMA)为溶解介质。结果5种有机溶剂完全分离,在所考察的浓度范围内具有良好的线性,r为0.9939-0.9999,甲醇、乙醇、丙酮、二氯甲烷、N,N-二甲基甲酰胺(DMF)的最低检测限分别为0.875,1.338,1.132,1.528,10.561,μg/mL,精密度RSD均小于2%,平均回收率为99.15%-103.32%.结论本实验建立的方法简便灵敏,结果准确可靠,可用于灯盏花乙素中有机溶剂残留量的检测。
Objective To establish a method for the determination of five residual solvents in seutellarin. Methods Head- space gas chromatography was established, the chromatographic column was DB-1301 (30mx0.32mmxl.0μm), the c, arrier gas was N2, the detector was FID, temperature was controlled by program and the solvent was N, N-Dimethylacetamide(DMA). Results Five residual solvents were completely separated. A good linearity (r=0.9939-0.9999)was obtained. The detection limits of methanol, ethanol, acetone, dichloromethane and DMF were 0.875, 1.338, 1.132, 1.528, 10.561μg'mL-1 respectively;the RSDs of precision were less than 2%; the average recovery rates of the preparation were in the range of 99.15%-103.32%. Conclusion The method is simple, sensitive, accurate and can be used for the quality control of scutellarin.
出处
《云南中医学院学报》
2014年第1期15-18,共4页
Journal of Yunnan University of Traditional Chinese Medicine